Comparison of responses elicited by immunization with a legionella species common lipoprotein delivered as naked DNA or recombinant protein

Suck Yoon Won, Hee Park Seol, Keun Park Yong, Chul Park Seung, Im Sin Jeong, Ja Kim Min

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD191+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalDNA and Cell Biology
Volume21
Issue number2
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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