Comparison of responses elicited by immunization with a legionella species common lipoprotein delivered as naked DNA or recombinant protein

Suck Yoon Won, Hee Park Seol, Keun Park Yong, Chul Park Seung, Im Sin Jeong, Ja Kim Min

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD191+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalDNA and Cell Biology
Volume21
Issue number2
DOIs
Publication statusPublished - 2002 Apr 22

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Legionella
Peptidoglycan
Recombinant Proteins
Lipoproteins
Immunization
B-Lymphocytes
Immunoglobulin G
Antigens
Legionella pneumophila
DNA Vaccines
Cytotoxic T-Lymphocytes
Antibody Formation
Vaccination
Proteins
Vaccines

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Comparison of responses elicited by immunization with a legionella species common lipoprotein delivered as naked DNA or recombinant protein. / Won, Suck Yoon; Seol, Hee Park; Yong, Keun Park; Seung, Chul Park; Jeong, Im Sin; Min, Ja Kim.

In: DNA and Cell Biology, Vol. 21, No. 2, 22.04.2002, p. 99-107.

Research output: Contribution to journalArticle

Won, Suck Yoon ; Seol, Hee Park ; Yong, Keun Park ; Seung, Chul Park ; Jeong, Im Sin ; Min, Ja Kim. / Comparison of responses elicited by immunization with a legionella species common lipoprotein delivered as naked DNA or recombinant protein. In: DNA and Cell Biology. 2002 ; Vol. 21, No. 2. pp. 99-107.
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