Abstract
To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD191+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.
| Original language | English |
|---|---|
| Pages (from-to) | 99-107 |
| Number of pages | 9 |
| Journal | DNA and Cell Biology |
| Volume | 21 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2002 Apr 22 |
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ASJC Scopus subject areas
- Cell Biology
- Genetics
- Molecular Biology
Cite this
Comparison of responses elicited by immunization with a legionella species common lipoprotein delivered as naked DNA or recombinant protein. / Won, Suck Yoon; Seol, Hee Park; Yong, Keun Park; Seung, Chul Park; Jeong, Im Sin; Min, Ja Kim.
In: DNA and Cell Biology, Vol. 21, No. 2, 22.04.2002, p. 99-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of responses elicited by immunization with a legionella species common lipoprotein delivered as naked DNA or recombinant protein
AU - Won, Suck Yoon
AU - Seol, Hee Park
AU - Yong, Keun Park
AU - Seung, Chul Park
AU - Jeong, Im Sin
AU - Min, Ja Kim
PY - 2002/4/22
Y1 - 2002/4/22
N2 - To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD191+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.
AB - To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD191+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036230469&partnerID=8YFLogxK
U2 - 10.1089/104454902753604970
DO - 10.1089/104454902753604970
M3 - Article
C2 - 11953009
AN - SCOPUS:0036230469
VL - 21
SP - 99
EP - 107
JO - DNA and Cell Biology
JF - DNA and Cell Biology
SN - 1044-5498
IS - 2
ER -