Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods

Jang Su Kim, Myung-Hyun Nam, Seong Soo A An, Chae Seung Lim, Dae Sung Hur, Chanil Chung, Jun Koon Chang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cell viability test is an essential tool in any laboratory, performing cell-based studies and clinical laboratory tests. The trypan blue exclusion method is the most popular assay for its simple concept among various diagnostic tools. However, several disadvantages include time-consuming and labor-intensive steps with low precision. In this study, we evaluated a new technique for the automatic cell viability measurement with microscopic cell counter and microchip. Upon blood draw from 11 healthy volunteers, Mononuclear cells were separated immediately from the heparinized whole blood, and the viable cells were diluted from 100 to 1%. The cell viability tests were performed simultaneously with following three methods: the conventional manual trypan blue exclusion method; the flow cytometry measurement with propidium iodide stain; and the newly developed microscopic cell counter with microchip. Linearities, precisions, and correlations from three methods were analyzed and compared. The correlations data from the microscopic cell counter were in good agreement with both the conventional trypan blue method (r=0.99, P<0.05) and the flow cytometry (r=0.99, P<0.05), respectively. The precision (2.0-6.2%) and linearity from the microscopic cell counter method with microchip were superior in comparison with the conventional method. The microscopic cell counter with microchip performed well with high precision, linearity, and efficient running time than both the manual trypan blue and the flow cytometry methods.

Original languageEnglish
Pages (from-to)90-94
Number of pages5
JournalJournal of Clinical Laboratory Analysis
Volume25
Issue number2
DOIs
Publication statusPublished - 2011 Mar 21
Externally publishedYes

Keywords

  • Flow cytometry
  • Microchip
  • Microscopic cell counter
  • Propidium iodide
  • Trypan blue
  • Viability

ASJC Scopus subject areas

  • Medical Laboratory Technology
  • Microbiology (medical)
  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Biochemistry, medical
  • Clinical Biochemistry

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