Comparison of the Diversity of Basidiomycetes from Dead Wood of the Manchurian fir (Abies holophylla) as Evaluated by Fruiting Body Collection, Mycelial Isolation, and 454 Sequencing

Yeongseon Jang, Seokyoon Jang, Mihee Min, Joo Hyun Hong, Hanbyul Lee, Hwanhwi Lee, Young Woon Lim, Jae-Jin Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this study, three different methods (fruiting body collection, mycelial isolation, and 454 sequencing) were implemented to determine the diversity of wood-inhabiting basidiomycetes from dead Manchurian fir (Abies holophylla). The three methods recovered similar species richness (26 species from fruiting bodies, 32 species from mycelia, and 32 species from 454 sequencing), but Fisher’s alpha, Shannon-Wiener, Simpson’s diversity indices of fungal communities indicated fruiting body collection and mycelial isolation displayed higher diversity compared with 454 sequencing. In total, 75 wood-inhabiting basidiomycetes were detected. The most frequently observed species were Heterobasidion orientale (fruiting body collection), Bjerkandera adusta (mycelial isolation), and Trichaptum fusco-violaceum (454 sequencing). Only two species, Hymenochaete yasudae and Hypochnicium karstenii, were detected by all three methods. This result indicated that Manchurian fir harbors a diverse basidiomycetous fungal community and for complete estimation of fungal diversity, multiple methods should be used. Further studies are required to understand their ecology in the context of forest ecosystems.

Original languageEnglish
Pages (from-to)634-645
Number of pages12
JournalMicrobial Ecology
Volume70
Issue number3
DOIs
Publication statusPublished - 2015 Oct 1

Fingerprint

Abies holophylla
dead wood
fruiting
fruiting bodies
Basidiomycota
fungal communities
Hypochnicium
Hymenochaete
Trichaptum
Bjerkandera adusta
Heterobasidion
methodology
forest ecosystems
mycelium
diversity index
forest ecosystem
ecology
harbor
species diversity
species richness

Keywords

  • Dead wood
  • Mycoparasitic fungi
  • Mycorrhizal fungi
  • Wood-decay fungi

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

Cite this

Comparison of the Diversity of Basidiomycetes from Dead Wood of the Manchurian fir (Abies holophylla) as Evaluated by Fruiting Body Collection, Mycelial Isolation, and 454 Sequencing. / Jang, Yeongseon; Jang, Seokyoon; Min, Mihee; Hong, Joo Hyun; Lee, Hanbyul; Lee, Hwanhwi; Lim, Young Woon; Kim, Jae-Jin.

In: Microbial Ecology, Vol. 70, No. 3, 01.10.2015, p. 634-645.

Research output: Contribution to journalArticle

Jang, Yeongseon ; Jang, Seokyoon ; Min, Mihee ; Hong, Joo Hyun ; Lee, Hanbyul ; Lee, Hwanhwi ; Lim, Young Woon ; Kim, Jae-Jin. / Comparison of the Diversity of Basidiomycetes from Dead Wood of the Manchurian fir (Abies holophylla) as Evaluated by Fruiting Body Collection, Mycelial Isolation, and 454 Sequencing. In: Microbial Ecology. 2015 ; Vol. 70, No. 3. pp. 634-645.
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