Abstract
The pressure during hyperbaric oxygen treatment may increase oxygen toxicity via an augmented oxygen pressure in the gas. Nevertheless, only a few reports have been published on the effect of cells grown under 2 atmospheric absolute (ATA) pressure. To evaluate the effect of pressure on oxygen toxicity and to study effects in addition to oxygen toxicity, we designed an experiment to compare the effects of normobaric mild hyperoxia (NMH, 40% oxygen) and hyperbaric air condition (HA, air with 2 ATA) on human diploid fibroblasts (HDF) in a hyperbaric incubator. HDFs in both the NMH and the HA condition had a similar oxidative stress response and exhibited premature senescence. To investigate differences in gene profiling in cells grown in the NMH and HA conditions, samples from cells exposed to each condition were applied to microarrays. We found no expression difference in genes related to aging and deoxyribonucleic acid damage, but the expression of genes including cell adhesion, stress response, and transcription were significantly increased in fibroblasts that were responsive to pressure. Among 26 statistically reliable genes, the expression of apoptosis related genes such as ADAM22, Bax, BCL2L14, and UBD, as well as tumor suppressor-related genes like Axin2 and ATF, and also mitogen-activated protein kinase-related genes like mitogen-activated protein kinase kinase kinase 1, histamine receptor, and RAB24, were significantly changed in cells responsive to pressureinduced oxidative stress.
| Original language | English |
|---|---|
| Pages (from-to) | 447-458 |
| Number of pages | 12 |
| Journal | Cell Stress and Chaperones |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2008 Dec 1 |
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Keywords
- Atmospheric pressure
- Heat shock protein
- Hyperbaric oxygenation
- Hyperoxia
- Microarray
- Real time RT-PCR
- Stress-induced premature senescence
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
Cite this
Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts. / Oh, Sangnam; Lee, Eun Il; Lee, Joohyun; Lim, Yongchul; Kim, Joonhee; Woo, Samyong.
In: Cell Stress and Chaperones, Vol. 13, No. 4, 01.12.2008, p. 447-458.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of the effects of 40% oxygen and two atmospheric absolute air pressure conditions on stress-induced premature senescence of normal human diploid fibroblasts
AU - Oh, Sangnam
AU - Lee, Eun Il
AU - Lee, Joohyun
AU - Lim, Yongchul
AU - Kim, Joonhee
AU - Woo, Samyong
PY - 2008/12/1
Y1 - 2008/12/1
N2 - The pressure during hyperbaric oxygen treatment may increase oxygen toxicity via an augmented oxygen pressure in the gas. Nevertheless, only a few reports have been published on the effect of cells grown under 2 atmospheric absolute (ATA) pressure. To evaluate the effect of pressure on oxygen toxicity and to study effects in addition to oxygen toxicity, we designed an experiment to compare the effects of normobaric mild hyperoxia (NMH, 40% oxygen) and hyperbaric air condition (HA, air with 2 ATA) on human diploid fibroblasts (HDF) in a hyperbaric incubator. HDFs in both the NMH and the HA condition had a similar oxidative stress response and exhibited premature senescence. To investigate differences in gene profiling in cells grown in the NMH and HA conditions, samples from cells exposed to each condition were applied to microarrays. We found no expression difference in genes related to aging and deoxyribonucleic acid damage, but the expression of genes including cell adhesion, stress response, and transcription were significantly increased in fibroblasts that were responsive to pressure. Among 26 statistically reliable genes, the expression of apoptosis related genes such as ADAM22, Bax, BCL2L14, and UBD, as well as tumor suppressor-related genes like Axin2 and ATF, and also mitogen-activated protein kinase-related genes like mitogen-activated protein kinase kinase kinase 1, histamine receptor, and RAB24, were significantly changed in cells responsive to pressureinduced oxidative stress.
AB - The pressure during hyperbaric oxygen treatment may increase oxygen toxicity via an augmented oxygen pressure in the gas. Nevertheless, only a few reports have been published on the effect of cells grown under 2 atmospheric absolute (ATA) pressure. To evaluate the effect of pressure on oxygen toxicity and to study effects in addition to oxygen toxicity, we designed an experiment to compare the effects of normobaric mild hyperoxia (NMH, 40% oxygen) and hyperbaric air condition (HA, air with 2 ATA) on human diploid fibroblasts (HDF) in a hyperbaric incubator. HDFs in both the NMH and the HA condition had a similar oxidative stress response and exhibited premature senescence. To investigate differences in gene profiling in cells grown in the NMH and HA conditions, samples from cells exposed to each condition were applied to microarrays. We found no expression difference in genes related to aging and deoxyribonucleic acid damage, but the expression of genes including cell adhesion, stress response, and transcription were significantly increased in fibroblasts that were responsive to pressure. Among 26 statistically reliable genes, the expression of apoptosis related genes such as ADAM22, Bax, BCL2L14, and UBD, as well as tumor suppressor-related genes like Axin2 and ATF, and also mitogen-activated protein kinase-related genes like mitogen-activated protein kinase kinase kinase 1, histamine receptor, and RAB24, were significantly changed in cells responsive to pressureinduced oxidative stress.
KW - Atmospheric pressure
KW - Heat shock protein
KW - Hyperbaric oxygenation
KW - Hyperoxia
KW - Microarray
KW - Real time RT-PCR
KW - Stress-induced premature senescence
UR - http://www.scopus.com/inward/record.url?scp=59649123039&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59649123039&partnerID=8YFLogxK
U2 - 10.1007/s12192-008-0041-5
DO - 10.1007/s12192-008-0041-5
M3 - Article
C2 - 18465208
AN - SCOPUS:59649123039
VL - 13
SP - 447
EP - 458
JO - Cell Stress and Chaperones
JF - Cell Stress and Chaperones
SN - 1355-8145
IS - 4
ER -