Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysis

Ju Han Song, Jong Man Kim, Seung Hyun Kim, Hyeoung Joon Kim, Jae Jung Lee, Myung Hui Sung, Seung Yong Hwang, Tae Sung Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

It is now recognized that precise patterns of differentially expressed genes ultimately direct a particular cell toward a given lineage. In this study, we compared the expression profiles of cancer-related genes by cDNA microarray analysis during the differentiation of human promyelocytic leukemia HL-60 cells into either monocytes or granulocytes. RNA was isolated at times 0, 6, 12, 24, 36, 48, and 72 h following stimulation of differentiation with all-trans retinoic acid (all-trans RA) or 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], and hybridized to the microarray gene chips containing 872 genes related to cell-cycles, oncogenes and leukemias. Several genes were commonly or differentially regulated during cell differentiation into either lineage, as demonstrated by both hierarchical and self-organizing map clustering analysis. At 72 h the expression levels of 45 genes were commonly up- or down-regulated at least a twofold in both lineages. Most importantly, 32 genes including α-L-fucosidase gene and adducin γ subunit gene were up- or down-regulated only in all-trans RA-treated HL-60 cells, while 12 genes including interleukin 1β and hypoxia-inducible factor 1α were up- or down-regulated only in 1,25-(OH)2D3-treated HL-60 cells. The expression of selected genes was confirmed by Northern blot analysis. As expected, some genes identified have not been examined during HL-60 cell differentiation into either lineage. The identification of genes associated with a specific differentiation lineage may give important insights into functional and phenotypic differences between two lineages of HL-60 cell differentiation.

Original languageEnglish
Pages (from-to)1705-1719
Number of pages15
JournalLife Sciences
Volume73
Issue number13
DOIs
Publication statusPublished - 2003 Aug 15
Externally publishedYes

Fingerprint

HL-60 Cells
Microarray Analysis
Microarrays
Oligonucleotide Array Sequence Analysis
Transcriptome
Gene expression
Cell Differentiation
Leukemia
Genes
DNA
Tretinoin
alpha-L-Fucosidase
Hypoxia-Inducible Factor 1
Calcitriol
Neoplasm Genes
Interleukin-1
Oncogenes
Granulocytes
Northern Blotting
Cluster Analysis

Keywords

  • 1,25-dihydroxyvitamin D
  • All-trans retinoic acid
  • Differentiation
  • Leukemia
  • Microarray

ASJC Scopus subject areas

  • Pharmacology

Cite this

Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysis. / Song, Ju Han; Kim, Jong Man; Kim, Seung Hyun; Kim, Hyeoung Joon; Lee, Jae Jung; Sung, Myung Hui; Hwang, Seung Yong; Kim, Tae Sung.

In: Life Sciences, Vol. 73, No. 13, 15.08.2003, p. 1705-1719.

Research output: Contribution to journalArticle

Song, Ju Han ; Kim, Jong Man ; Kim, Seung Hyun ; Kim, Hyeoung Joon ; Lee, Jae Jung ; Sung, Myung Hui ; Hwang, Seung Yong ; Kim, Tae Sung. / Comparison of the gene expression profiles of monocytic versus granulocytic lineages of HL-60 leukemia cell differentiation by DNA microarray analysis. In: Life Sciences. 2003 ; Vol. 73, No. 13. pp. 1705-1719.
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