Comparison of the mismatch repair system between primary and metastatic colorectal cancers using immunohistochemistry

Jiyoon Jung, Youngjin Kang, Yoo Jin Lee, Eojin Kim, Bokyung Ahn, Eunjung Lee, Joo Young Kim, Jeong Hyeon Lee, Youngseok Lee, Chul Hwan Kim, Yang Seok Chae

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Colorectal cancer (CRC) is one of the most common malignancies worldwide. Approximately 10%-15% of the CRC cases have defective DNA mismatch repair (MMR) genes. Although the high level of microsatellite instability status is a predictor of favorable outcome in primary CRC, little is known about its frequency and importance in secondary CRC. Immunohistochemical staining (IHC) for MMR proteins (e.g., MLH1, MSH2, MSH6, and PMS2) has emerged as a useful technique to complement polymerase chain reaction (PCR) analyses. Methods: In this study, comparison between the MMR system of primary CRCs and paired liver and lung metastatic lesions was done using IHC and the correlation with clinical outcomes was also examined. Results: Based on IHC, 7/61 primary tumors (11.4%) showed deficient MMR systems, while 13/61 secondary tumors (21.3%) showed deficiencies. In total, 44 cases showed proficient expression in both the primary and metastatic lesions. Three cases showed deficiencies in both the primary and paired metastatic lesions. In 10 cases, proficient expression was found only in the primary lesions, and not in the corresponding metastatic lesions. In four cases, proficient expression was detected in the secondary tumor, but not in the primary tumor. Conclusions: Although each IHC result and the likely defective genes were not exactly matched between the primary and the metastatic tumors, identical results for primary and metastatic lesions were obtained in 77% of the cases (47/61). These data are in agreement with the previous microsatellite detection studies that used PCR and IHC.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalJournal of Pathology and Translational Medicine
Volume51
Issue number2
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

DNA Mismatch Repair
Colorectal Neoplasms
Immunohistochemistry
Staining and Labeling
Neoplasms
Polymerase Chain Reaction
Microsatellite Instability
Microsatellite Repeats
Genes
Lung
Liver

Keywords

  • Colorectal neoplasms
  • DNA mismatch repair
  • Immunohistochemistry
  • Microsatellite instability

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Comparison of the mismatch repair system between primary and metastatic colorectal cancers using immunohistochemistry. / Jung, Jiyoon; Kang, Youngjin; Lee, Yoo Jin; Kim, Eojin; Ahn, Bokyung; Lee, Eunjung; Kim, Joo Young; Lee, Jeong Hyeon; Lee, Youngseok; Kim, Chul Hwan; Chae, Yang Seok.

In: Journal of Pathology and Translational Medicine, Vol. 51, No. 2, 01.01.2017, p. 129-136.

Research output: Contribution to journalArticle

Jung, Jiyoon ; Kang, Youngjin ; Lee, Yoo Jin ; Kim, Eojin ; Ahn, Bokyung ; Lee, Eunjung ; Kim, Joo Young ; Lee, Jeong Hyeon ; Lee, Youngseok ; Kim, Chul Hwan ; Chae, Yang Seok. / Comparison of the mismatch repair system between primary and metastatic colorectal cancers using immunohistochemistry. In: Journal of Pathology and Translational Medicine. 2017 ; Vol. 51, No. 2. pp. 129-136.
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abstract = "Background: Colorectal cancer (CRC) is one of the most common malignancies worldwide. Approximately 10{\%}-15{\%} of the CRC cases have defective DNA mismatch repair (MMR) genes. Although the high level of microsatellite instability status is a predictor of favorable outcome in primary CRC, little is known about its frequency and importance in secondary CRC. Immunohistochemical staining (IHC) for MMR proteins (e.g., MLH1, MSH2, MSH6, and PMS2) has emerged as a useful technique to complement polymerase chain reaction (PCR) analyses. Methods: In this study, comparison between the MMR system of primary CRCs and paired liver and lung metastatic lesions was done using IHC and the correlation with clinical outcomes was also examined. Results: Based on IHC, 7/61 primary tumors (11.4{\%}) showed deficient MMR systems, while 13/61 secondary tumors (21.3{\%}) showed deficiencies. In total, 44 cases showed proficient expression in both the primary and metastatic lesions. Three cases showed deficiencies in both the primary and paired metastatic lesions. In 10 cases, proficient expression was found only in the primary lesions, and not in the corresponding metastatic lesions. In four cases, proficient expression was detected in the secondary tumor, but not in the primary tumor. Conclusions: Although each IHC result and the likely defective genes were not exactly matched between the primary and the metastatic tumors, identical results for primary and metastatic lesions were obtained in 77{\%} of the cases (47/61). These data are in agreement with the previous microsatellite detection studies that used PCR and IHC.",
keywords = "Colorectal neoplasms, DNA mismatch repair, Immunohistochemistry, Microsatellite instability",
author = "Jiyoon Jung and Youngjin Kang and Lee, {Yoo Jin} and Eojin Kim and Bokyung Ahn and Eunjung Lee and Kim, {Joo Young} and Lee, {Jeong Hyeon} and Youngseok Lee and Kim, {Chul Hwan} and Chae, {Yang Seok}",
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AU - Jung, Jiyoon

AU - Kang, Youngjin

AU - Lee, Yoo Jin

AU - Kim, Eojin

AU - Ahn, Bokyung

AU - Lee, Eunjung

AU - Kim, Joo Young

AU - Lee, Jeong Hyeon

AU - Lee, Youngseok

AU - Kim, Chul Hwan

AU - Chae, Yang Seok

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N2 - Background: Colorectal cancer (CRC) is one of the most common malignancies worldwide. Approximately 10%-15% of the CRC cases have defective DNA mismatch repair (MMR) genes. Although the high level of microsatellite instability status is a predictor of favorable outcome in primary CRC, little is known about its frequency and importance in secondary CRC. Immunohistochemical staining (IHC) for MMR proteins (e.g., MLH1, MSH2, MSH6, and PMS2) has emerged as a useful technique to complement polymerase chain reaction (PCR) analyses. Methods: In this study, comparison between the MMR system of primary CRCs and paired liver and lung metastatic lesions was done using IHC and the correlation with clinical outcomes was also examined. Results: Based on IHC, 7/61 primary tumors (11.4%) showed deficient MMR systems, while 13/61 secondary tumors (21.3%) showed deficiencies. In total, 44 cases showed proficient expression in both the primary and metastatic lesions. Three cases showed deficiencies in both the primary and paired metastatic lesions. In 10 cases, proficient expression was found only in the primary lesions, and not in the corresponding metastatic lesions. In four cases, proficient expression was detected in the secondary tumor, but not in the primary tumor. Conclusions: Although each IHC result and the likely defective genes were not exactly matched between the primary and the metastatic tumors, identical results for primary and metastatic lesions were obtained in 77% of the cases (47/61). These data are in agreement with the previous microsatellite detection studies that used PCR and IHC.

AB - Background: Colorectal cancer (CRC) is one of the most common malignancies worldwide. Approximately 10%-15% of the CRC cases have defective DNA mismatch repair (MMR) genes. Although the high level of microsatellite instability status is a predictor of favorable outcome in primary CRC, little is known about its frequency and importance in secondary CRC. Immunohistochemical staining (IHC) for MMR proteins (e.g., MLH1, MSH2, MSH6, and PMS2) has emerged as a useful technique to complement polymerase chain reaction (PCR) analyses. Methods: In this study, comparison between the MMR system of primary CRCs and paired liver and lung metastatic lesions was done using IHC and the correlation with clinical outcomes was also examined. Results: Based on IHC, 7/61 primary tumors (11.4%) showed deficient MMR systems, while 13/61 secondary tumors (21.3%) showed deficiencies. In total, 44 cases showed proficient expression in both the primary and metastatic lesions. Three cases showed deficiencies in both the primary and paired metastatic lesions. In 10 cases, proficient expression was found only in the primary lesions, and not in the corresponding metastatic lesions. In four cases, proficient expression was detected in the secondary tumor, but not in the primary tumor. Conclusions: Although each IHC result and the likely defective genes were not exactly matched between the primary and the metastatic tumors, identical results for primary and metastatic lesions were obtained in 77% of the cases (47/61). These data are in agreement with the previous microsatellite detection studies that used PCR and IHC.

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