Comparison of the novel human papillomavirus 4 auto-capillary electrophoresis test with the hybrid capture 2 assay and with the PCR HPV typing set test in the detection of high-risk hpv including HPV 16 and 18 genotypes in cervical specimens

Jin-Hwa Hong, Seung Hun Song, Jong Kee Kim, Jeong Hyun Han, Jae Kwan Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The aim of this study was to compare the novel human papillomavirus (HPV) detection method, the HPV 4 Auto-capillary Electrophoresis (ACE) test with the hybrid capture (HC) 2 assay for the detection of high-risk HPVs. In addition, we compared the HPV 4 ACE test with the polymerase chain reaction HPV Typing Set test for the detection of HPV 16 and HPV 18 genotypes. One hundred ninety-nine cervical swab samples obtained from women with previous abnormal Pap smears were subjected to testing with the three HPV tests. The HPV 4 ACE test and the HC 2 assay showed substantial agreement for detection of high-risk HPVs (85.4%, kappa=0.71). The HPV 4 ACE test also showed substantial agreement with the PCR HPV Typing Set test in the detection of HPV 16 and HP V 18 genotypes (89.9%, kappa=0.65). In correlation with cytologic results, the sensitivities and specificities of the HPV 4 ACE test and HC 2 assay were 92.9% vs. 92.9% and 48.1% vs. 50.8%, respectively, when high-grade squamous intraepithelial lesions were regarded as abnormal cytologies. The novel HPV 4 ACE test is a valuable tool for the detection of high-risk HPVs and for genotyping of HPV 16 and HPV 18.

Original languageEnglish
Pages (from-to)579-584
Number of pages6
JournalJournal of Korean Medical Science
Volume24
Issue number4
DOIs
Publication statusPublished - 2009 Aug 1

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Gammapapillomavirus
Human papillomavirus 18
Human papillomavirus 16
Capillary Electrophoresis
Genotype
Polymerase Chain Reaction
Papanicolaou Test
Cell Biology
Sensitivity and Specificity

Keywords

  • ACE
  • HC2
  • HPV typing methods
  • HPV typing set test

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Comparison of the novel human papillomavirus 4 auto-capillary electrophoresis test with the hybrid capture 2 assay and with the PCR HPV typing set test in the detection of high-risk hpv including HPV 16 and 18 genotypes in cervical specimens",
abstract = "The aim of this study was to compare the novel human papillomavirus (HPV) detection method, the HPV 4 Auto-capillary Electrophoresis (ACE) test with the hybrid capture (HC) 2 assay for the detection of high-risk HPVs. In addition, we compared the HPV 4 ACE test with the polymerase chain reaction HPV Typing Set test for the detection of HPV 16 and HPV 18 genotypes. One hundred ninety-nine cervical swab samples obtained from women with previous abnormal Pap smears were subjected to testing with the three HPV tests. The HPV 4 ACE test and the HC 2 assay showed substantial agreement for detection of high-risk HPVs (85.4{\%}, kappa=0.71). The HPV 4 ACE test also showed substantial agreement with the PCR HPV Typing Set test in the detection of HPV 16 and HP V 18 genotypes (89.9{\%}, kappa=0.65). In correlation with cytologic results, the sensitivities and specificities of the HPV 4 ACE test and HC 2 assay were 92.9{\%} vs. 92.9{\%} and 48.1{\%} vs. 50.8{\%}, respectively, when high-grade squamous intraepithelial lesions were regarded as abnormal cytologies. The novel HPV 4 ACE test is a valuable tool for the detection of high-risk HPVs and for genotyping of HPV 16 and HPV 18.",
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author = "Jin-Hwa Hong and Song, {Seung Hun} and Kim, {Jong Kee} and Han, {Jeong Hyun} and Lee, {Jae Kwan}",
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AU - Lee, Jae Kwan

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