Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio

Eun Jung Park, Gwang Hee Lee, Jae hun Shim, Myung Haing Cho, Byoung Seok Lee, Yong Bum Kim, Jae Ho Kim, Younghun Kim, Dong-Wan Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aluminum oxide nanoparticles are listed among 14 high-priority nanomaterials published by the Organization for Economic Co-operation and Development, but limited information is available on their potential hazards. In this study, we compared the toxicity of two different aluminum oxide nanorods (AlNRs) commercially available in vivo and in vitro. Considering aspect ratio, one was 6.2 ± 0.6 (long-AlNRs) and the other was 2.1 ± 0.4 (short-AlNRs). In mice, long-AlNRs induced longer and stronger inflammatory responses than short-AlNRs, and the degree reached the maximum on day 7 for both types and decreased with time. In addition, in vitro tests were performed on six cell lines derived from potential target organs for AlNPs, HEK-293 (kidney), HACAT (skin), Chang (liver), BEAS-2B (lung), T98G (brain), and H9C2 (heart), using MTT assay, ATP assay, LDH release, and xCELLigence system. Long-AlNRs generally produced stronger toxicity than short-AlNRs, and HEK-293 cells were the most sensitive for both AlNRs, followed by BEAS-2B cells, although results from 4 kinds of toxicity tests conflicted among the cell lines. Based on these results, we suggest that toxicity of AlNRs may be related to aspect ratio (and resultant surface area). Furthermore, novel in vitro toxicity testing methods are needed to resolve questionable results caused by the unique properties of nanoparticles.

Original languageEnglish
Pages (from-to)1771-1782
Number of pages12
JournalArchives of Toxicology
Volume89
Issue number10
DOIs
Publication statusPublished - 2015 Oct 24

Fingerprint

Nanotubes
Aluminum Oxide
Nanorods
Toxicity
Aspect ratio
Nanoparticles
Assays
Cells
Cell Line
Toxicity Tests
Nanostructures
HEK293 Cells
Nanostructured materials
Liver
Brain
Hazards
Skin
Adenosine Triphosphate
Kidney

Keywords

  • Aluminum oxide nanoparticles
  • Aspect ratio
  • In vitro system
  • Nanorods
  • Toxicity

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio. / Park, Eun Jung; Lee, Gwang Hee; Shim, Jae hun; Cho, Myung Haing; Lee, Byoung Seok; Kim, Yong Bum; Kim, Jae Ho; Kim, Younghun; Kim, Dong-Wan.

In: Archives of Toxicology, Vol. 89, No. 10, 24.10.2015, p. 1771-1782.

Research output: Contribution to journalArticle

Park, EJ, Lee, GH, Shim, JH, Cho, MH, Lee, BS, Kim, YB, Kim, JH, Kim, Y & Kim, D-W 2015, 'Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio', Archives of Toxicology, vol. 89, no. 10, pp. 1771-1782. https://doi.org/10.1007/s00204-014-1332-5
Park, Eun Jung ; Lee, Gwang Hee ; Shim, Jae hun ; Cho, Myung Haing ; Lee, Byoung Seok ; Kim, Yong Bum ; Kim, Jae Ho ; Kim, Younghun ; Kim, Dong-Wan. / Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio. In: Archives of Toxicology. 2015 ; Vol. 89, No. 10. pp. 1771-1782.
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