Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV

Seung Gyu Yun, Min Young Kim, Jong Moon Choi, Chang Kyu Lee, Chae Seung Lim, Yunjung Cho, In Bum Suh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.

Original languageEnglish
JournalJournal of Clinical Laboratory Analysis
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Multiplex Polymerase Chain Reaction
Viruses
Assays
Nucleic Acid Amplification Techniques
Sputum
Nucleic Acids
Amplification
Polymerase Chain Reaction

Keywords

  • Agreement
  • Nucleic acid amplification test
  • Respiratory virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

Cite this

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title = "Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV",
abstract = "Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2{\%}), 99 (49.3{\%}), and 95 (47.3{\%}) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97{\%}-98{\%}, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94{\%}-100{\%} agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.",
keywords = "Agreement, Nucleic acid amplification test, Respiratory virus",
author = "Yun, {Seung Gyu} and Kim, {Min Young} and Choi, {Jong Moon} and Lee, {Chang Kyu} and Lim, {Chae Seung} and Yunjung Cho and Suh, {In Bum}",
year = "2017",
doi = "10.1002/jcla.22230",
language = "English",
journal = "Journal of Clinical Laboratory Analysis",
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T1 - Comparison of three multiplex PCR assays for detection of respiratory viruses

T2 - Anyplex II RV16, AdvanSure RV, and Real-Q RV

AU - Yun, Seung Gyu

AU - Kim, Min Young

AU - Choi, Jong Moon

AU - Lee, Chang Kyu

AU - Lim, Chae Seung

AU - Cho, Yunjung

AU - Suh, In Bum

PY - 2017

Y1 - 2017

N2 - Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.

AB - Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.

KW - Agreement

KW - Nucleic acid amplification test

KW - Respiratory virus

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