TY - JOUR
T1 - Comparison of three multiplex PCR assays for detection of respiratory viruses
T2 - Anyplex II RV16, AdvanSure RV, and Real-Q RV
AU - Yun, Seung Gyu
AU - Kim, Min Young
AU - Choi, Jong Moon
AU - Lee, Chang Kyu
AU - Lim, Chae Seung
AU - Cho, Yunjung
AU - Suh, In Bum
N1 - Funding Information:
This study was partly supported by LG Life science, Korea and Biosewoom, Korea through supplying AdvanSure RV and Real-Q RV kits. The sponsors had no involvement in the design, data interpretation and making of the manuscript. We thank LG Life Science, Biosewoom and Seegene for their technical assistance in the validation of the results. The authors also acknowledge the assistance of master sergeant Hyun-Ki Moon and warrant officer Kwon-Young Lee in Armed Forces Daejeon Hospital.
Publisher Copyright:
© 2017 The Authors Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc.
PY - 2018/2
Y1 - 2018/2
N2 - Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.
AB - Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.
KW - agreement
KW - nucleic acid amplification test
KW - respiratory virus
UR - http://www.scopus.com/inward/record.url?scp=85017559321&partnerID=8YFLogxK
U2 - 10.1002/jcla.22230
DO - 10.1002/jcla.22230
M3 - Article
C2 - 28397965
AN - SCOPUS:85017559321
VL - 32
JO - Journal of Clinical Laboratory Analysis
JF - Journal of Clinical Laboratory Analysis
SN - 0887-8013
IS - 2
M1 - e22230
ER -