Comparisons of phylogenetic hypotheses among different data sets in dwarf dandelions (Krigia, Asteraceae): Additional information from internal transcribed spacer sequences of nuclear ribosomal DNA

K. J. Kim, R. K. Jansen

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

The internal transcribed spacer (ITS) region of the 18 S-25 S nuclear ribosomal DNA repeat was sequenced from 19 populations of the tribe Lactuceae, including all species of dwarf dandelion (Krigia) and five outgroup genera. The incidence of length changes and base substitutions was at least two times higher for ITS 1 than ITS 2. Interspecific sequence divergence within Krigia averaged 9.62% (1.61%-15.19%) and 4.26% (0%-6.64%) in ITS 1 and ITS 2, respectively. Intergeneric sequence divergence ranged from 15.6% to 44.5% in ITS 1 and from 8.0% to 28.6% in ITS 2. High sequence divergence and homoplasy among genera of tribe Lactuceae suggest that the phylogenetic utility of ITS sequence data is limited to interspecific studies or comparisons among closely related genera. Trees generated from ITS sequences are essentially identical to those from restriction site comparisons of the entire nuclear ribosomal (nr) DNA region. The degree of tree resolution differed depending on how gaps were treated in phylogenetic analyses. The ITS trees were congruent with the chloroplast DNA and morphological phylogenies in three major ways: 1) the sister group relationship between Krigia and Pyrrhopappus; 2) the recognition of two monophyletic sections, Krigia and Cymbia, in genus Krigia; and 3) the monophyly of the K. occidentalis-K. cespitosa clade in sect. Cymbia. However, the two nrDNA-based trees are not congruent with morphology/chloroplast DNA-based trees for the interspecific relationships in sect. Krigia. An average of 22.5% incongruence was observed among four Krigia data sets. The relatively high degree of incongruence among data sets is due primarily to conflict between trees based on nrDNA and morphological/cpDNA data. The incongruence is probably due to the concerted evolution of nrDNA repeating units. The results from Krigia and the Lactuceae suggest that nrDNA data may have limited utility in phylogenetic studies of plants, especially in groups which exhibit high levels of sequence divergence. Our combined phylogenetic analysis as a total evidence shows the least conflict to each of the individual data sets.

Original languageEnglish
Pages (from-to)157-185
Number of pages29
JournalPlant Systematics and Evolution
Volume190
Issue number3-4
DOIs
Publication statusPublished - 1994 Sep

Keywords

  • Asteraceae
  • ITS region (ITS 1, 5.8 S rDNA, and ITS 2)
  • Internal transcribed spacer (ITS)
  • Krigia
  • Lactuceae
  • Microseridinae
  • chloroplast DNA (cpDNA)
  • intergenic spacer (IGS) region
  • maximum parsimony (MP) tree
  • neighbor joining (NJ) tree
  • nuclear ribosomal DNA (nrDNA)
  • polymerase chain reaction (PCR)
  • sequence divergence

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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