Compartmentalization and regulation of mitochondrial function by methionine sulfoxide reductases in yeast

Alaattin Kaya, Ahmet Koc, Byung Cheon Lee, Dmitri E. Fomenko, Mathieu Rederstorff, Alain Krol, Alain Lescure, Vadim N. Gladyshev

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Elevated levels of reactive oxygen species can damage proteins. Sulfur-containing amino acid residues, cysteine and methionine, are particularly susceptible to such damage. Various enzymes evolved to protect proteins or repair oxidized residues, including methionine sulfoxide reductases MsrA and MsrB, which reduce methionine (S)-sulfoxide (Met-SO) and methionine (R)-sulfoxide (Met-RO) residues, respectively, back to methionine. Here, we show that MsrA and MsrB are involved in the regulation of mitochondrial function. Saccharomyces cerevisiae mutant cells lacking MsrA, MsrB, or both proteins had normal levels of mitochondria but lower levels of cytochrome c and fewer respiration-competent mitochondria. The growth of single MsrA or MsrB mutants on respiratory carbon sources was inhibited, and that of the double mutant was severely compromised, indicating impairment of mitochondrial function. Although MsrA and MsrB are thought to have similar roles in oxidative protein repair each targeting a diastereomer of methionine sulfoxide, their deletion resulted in different phenotypes. GFP fusions of MsrA and MsrB showed different localization patterns and primarily localized to cytoplasm and mitochondria, respectively. This finding agreed with compartment-specific enrichment of MsrA and MsrB activities. These results show that oxidative stress contributes to mitochondrial dysfunction through oxidation of methionine residues in proteins located in different cellular compartments.

Original languageEnglish
Pages (from-to)8618-8625
Number of pages8
JournalBiochemistry
Volume49
Issue number39
DOIs
Publication statusPublished - 2010 Oct 5
Externally publishedYes

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Methionine Sulfoxide Reductases
sulfoxide
Methionine
Yeast
Yeasts
Mitochondria
Proteins
Repair
Sulfur Amino Acids
Oxidative stress
Cytochromes c
Sulfur
Cysteine
Saccharomyces cerevisiae
Reactive Oxygen Species
Respiration
Cytoplasm
Oxidative Stress
Fusion reactions
Carbon

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kaya, A., Koc, A., Lee, B. C., Fomenko, D. E., Rederstorff, M., Krol, A., ... Gladyshev, V. N. (2010). Compartmentalization and regulation of mitochondrial function by methionine sulfoxide reductases in yeast. Biochemistry, 49(39), 8618-8625. https://doi.org/10.1021/bi100908v

Compartmentalization and regulation of mitochondrial function by methionine sulfoxide reductases in yeast. / Kaya, Alaattin; Koc, Ahmet; Lee, Byung Cheon; Fomenko, Dmitri E.; Rederstorff, Mathieu; Krol, Alain; Lescure, Alain; Gladyshev, Vadim N.

In: Biochemistry, Vol. 49, No. 39, 05.10.2010, p. 8618-8625.

Research output: Contribution to journalArticle

Kaya, A, Koc, A, Lee, BC, Fomenko, DE, Rederstorff, M, Krol, A, Lescure, A & Gladyshev, VN 2010, 'Compartmentalization and regulation of mitochondrial function by methionine sulfoxide reductases in yeast', Biochemistry, vol. 49, no. 39, pp. 8618-8625. https://doi.org/10.1021/bi100908v
Kaya, Alaattin ; Koc, Ahmet ; Lee, Byung Cheon ; Fomenko, Dmitri E. ; Rederstorff, Mathieu ; Krol, Alain ; Lescure, Alain ; Gladyshev, Vadim N. / Compartmentalization and regulation of mitochondrial function by methionine sulfoxide reductases in yeast. In: Biochemistry. 2010 ; Vol. 49, No. 39. pp. 8618-8625.
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