Abstract
We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.
| Original language | English |
|---|---|
| Pages (from-to) | 987-994 |
| Number of pages | 8 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 74 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2007 Apr 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Microbiology
- Bioengineering
- Microbiology (medical)
Cite this
Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis : C/N ratio and light intensity. / Kang, C. D.; Lee, J. S.; Park, T. H.; Sim, Sang Jun.
In: Applied Microbiology and Biotechnology, Vol. 74, No. 5, 01.04.2007, p. 987-994.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis
T2 - C/N ratio and light intensity
AU - Kang, C. D.
AU - Lee, J. S.
AU - Park, T. H.
AU - Sim, Sang Jun
PY - 2007/4/1
Y1 - 2007/4/1
N2 - We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.
AB - We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.
UR - http://www.scopus.com/inward/record.url?scp=33947225848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947225848&partnerID=8YFLogxK
U2 - 10.1007/s00253-006-0759-x
DO - 10.1007/s00253-006-0759-x
M3 - Article
C2 - 17216459
AN - SCOPUS:33947225848
VL - 74
SP - 987
EP - 994
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -