Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis: C/N ratio and light intensity

C. D. Kang, J. S. Lee, T. H. Park, Sang Jun Sim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.

Original languageEnglish
Pages (from-to)987-994
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume74
Issue number5
DOIs
Publication statusPublished - 2007 Apr 1
Externally publishedYes

Fingerprint

Nitrogen
Carbon
Light
Photons
Biomass
astaxanthine
Cell Count
Air

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis : C/N ratio and light intensity. / Kang, C. D.; Lee, J. S.; Park, T. H.; Sim, Sang Jun.

In: Applied Microbiology and Biotechnology, Vol. 74, No. 5, 01.04.2007, p. 987-994.

Research output: Contribution to journalArticle

@article{dab416ea56894017b738a3d7117d8478,
title = "Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis: C/N ratio and light intensity",
abstract = "We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.",
author = "Kang, {C. D.} and Lee, {J. S.} and Park, {T. H.} and Sim, {Sang Jun}",
year = "2007",
month = "4",
day = "1",
doi = "10.1007/s00253-006-0759-x",
language = "English",
volume = "74",
pages = "987--994",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis

T2 - C/N ratio and light intensity

AU - Kang, C. D.

AU - Lee, J. S.

AU - Park, T. H.

AU - Sim, Sang Jun

PY - 2007/4/1

Y1 - 2007/4/1

N2 - We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.

AB - We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.

UR - http://www.scopus.com/inward/record.url?scp=33947225848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947225848&partnerID=8YFLogxK

U2 - 10.1007/s00253-006-0759-x

DO - 10.1007/s00253-006-0759-x

M3 - Article

C2 - 17216459

AN - SCOPUS:33947225848

VL - 74

SP - 987

EP - 994

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 5

ER -