TY - JOUR
T1 - Complementary limiting factors of astaxanthin synthesis during photoautotrophic induction of Haematococcus pluvialis
T2 - C/N ratio and light intensity
AU - Kang, C. D.
AU - Lee, J. S.
AU - Park, T. H.
AU - Sim, S. J.
N1 - Funding Information:
Acknowledgment This research was supported by a grant (DG2-201) from the Carbon Dioxide Reduction & Sequestration Research Center, one of the 21st Century Frontier Programs funded by the Ministry of Science and Technology of the Korean government.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/4
Y1 - 2007/4
N2 - We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.
AB - We investigated the effect of carbon/nitrogen (C/N) ratio on astaxanthin synthesis in Haematococcus pluvialis during photoautotrophic induction by continuous input of both CO2-air mixture and intense light. When H. pluvialis was induced by constant irradiance induction at 200 μmol photon m-2 s-1, there was a positive correlation with astaxanthin content and C/N ratio, which was similar to the case for heterotrophic induction. Lower C/N ratios did not retard Haematococcus encystment, but did increase culture biomass, resulting in a decrease in astaxanthin production because of light limitation. However, induction using variable irradiance showed that reduction of astaxanthin production at low C/N ratios was successfully overcome by simply increasing the light intensity from 200 to 300 μmol photon m-2 s-1 to overcome the light limitation. This resulted in a greatly enhanced astaxanthin synthesis in proportion to cell density in cultures with low C/N ratios. Our results indicate that light intensity is more critical than C/N ratio in astaxanthin production by H. pluvialis during photoautotrophic induction.
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U2 - 10.1007/s00253-006-0759-x
DO - 10.1007/s00253-006-0759-x
M3 - Article
C2 - 17216459
AN - SCOPUS:33947225848
VL - 74
SP - 987
EP - 994
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -