Complete genome sequence of a novel phage, vB_MoxS-ISF9, infecting methylotrophic Microbacterium

first report of a virulent Microbacterium phage.

Isaac Zamani, Majid Bouzari, Giti Emtiazi, Seyed Mahdi Ghasemi, Hyo-Ihl Chang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Here, we report the first genome sequence of a new virulent phage of Microbacterium oxydans, termed vB_MoxS-ISF9, which was isolated from sewage. Transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family Siphoviridae. The vB_MoxS-ISF9 DNA was completely sequenced and found to be 59,254 bp in length, with a G+C content of 62.76% and 120 putative open reading frames (ORFs). The predicted protein products of the ORFs were identified, and their sequences were analyzed. In a comparison with all available phage genomes, vB_MoxS-ISF9 did not show any significant similarity to other previously reported bacteriophages. To the beast of our knowledge, this is the first report of the isolation and complete genomic sequencing of a virulent phage against a member of the genus Microbacterium.

Original languageEnglish
Pages (from-to)2537-2540
Number of pages4
JournalArchives of Virology
Volume159
Issue number9
DOIs
Publication statusPublished - 2014 Jan 1

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Bacteriophages
Genes
Genome
Open Reading Frames
Siphoviridae
Base Composition
Sewage
Transmission Electron Microscopy
Head
Transmission electron microscopy
DNA
Proteins

ASJC Scopus subject areas

  • Virology

Cite this

Complete genome sequence of a novel phage, vB_MoxS-ISF9, infecting methylotrophic Microbacterium : first report of a virulent Microbacterium phage. / Zamani, Isaac; Bouzari, Majid; Emtiazi, Giti; Ghasemi, Seyed Mahdi; Chang, Hyo-Ihl.

In: Archives of Virology, Vol. 159, No. 9, 01.01.2014, p. 2537-2540.

Research output: Contribution to journalArticle

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