Abstract
Here, we report the first genome sequence of a new virulent phage of Microbacterium oxydans, termed vB_MoxS-ISF9, which was isolated from sewage. Transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family Siphoviridae. The vB_MoxS-ISF9 DNA was completely sequenced and found to be 59,254 bp in length, with a G+C content of 62.76% and 120 putative open reading frames (ORFs). The predicted protein products of the ORFs were identified, and their sequences were analyzed. In a comparison with all available phage genomes, vB_MoxS-ISF9 did not show any significant similarity to other previously reported bacteriophages. To the beast of our knowledge, this is the first report of the isolation and complete genomic sequencing of a virulent phage against a member of the genus Microbacterium.
| Original language | English |
|---|---|
| Pages (from-to) | 2537-2540 |
| Number of pages | 4 |
| Journal | Archives of Virology |
| Volume | 159 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
Fingerprint
ASJC Scopus subject areas
- Virology
Cite this
Complete genome sequence of a novel phage, vB_MoxS-ISF9, infecting methylotrophic Microbacterium : first report of a virulent Microbacterium phage. / Zamani, Isaac; Bouzari, Majid; Emtiazi, Giti; Ghasemi, Seyed Mahdi; Chang, Hyo-Ihl.
In: Archives of Virology, Vol. 159, No. 9, 01.01.2014, p. 2537-2540.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Complete genome sequence of a novel phage, vB_MoxS-ISF9, infecting methylotrophic Microbacterium
T2 - first report of a virulent Microbacterium phage.
AU - Zamani, Isaac
AU - Bouzari, Majid
AU - Emtiazi, Giti
AU - Ghasemi, Seyed Mahdi
AU - Chang, Hyo-Ihl
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Here, we report the first genome sequence of a new virulent phage of Microbacterium oxydans, termed vB_MoxS-ISF9, which was isolated from sewage. Transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family Siphoviridae. The vB_MoxS-ISF9 DNA was completely sequenced and found to be 59,254 bp in length, with a G+C content of 62.76% and 120 putative open reading frames (ORFs). The predicted protein products of the ORFs were identified, and their sequences were analyzed. In a comparison with all available phage genomes, vB_MoxS-ISF9 did not show any significant similarity to other previously reported bacteriophages. To the beast of our knowledge, this is the first report of the isolation and complete genomic sequencing of a virulent phage against a member of the genus Microbacterium.
AB - Here, we report the first genome sequence of a new virulent phage of Microbacterium oxydans, termed vB_MoxS-ISF9, which was isolated from sewage. Transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family Siphoviridae. The vB_MoxS-ISF9 DNA was completely sequenced and found to be 59,254 bp in length, with a G+C content of 62.76% and 120 putative open reading frames (ORFs). The predicted protein products of the ORFs were identified, and their sequences were analyzed. In a comparison with all available phage genomes, vB_MoxS-ISF9 did not show any significant similarity to other previously reported bacteriophages. To the beast of our knowledge, this is the first report of the isolation and complete genomic sequencing of a virulent phage against a member of the genus Microbacterium.
UR - http://www.scopus.com/inward/record.url?scp=84907879699&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907879699&partnerID=8YFLogxK
U2 - 10.1007/s00705-014-2092-5
DO - 10.1007/s00705-014-2092-5
M3 - Article
VL - 159
SP - 2537
EP - 2540
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 9
ER -