TY - JOUR
T1 - Complete genome sequence of a novel phage, vB_MoxS-ISF9, infecting methylotrophic Microbacterium
T2 - first report of a virulent Microbacterium phage.
AU - Zamani, Isaac
AU - Bouzari, Majid
AU - Emtiazi, Giti
AU - Ghasemi, Seyed Mahdi
AU - Chang, Hyo Ihl
N1 - Funding Information:
This research was funded by an operating grant from the Dean of Research and Graduate Studies at the University of Isfahan and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2012R1A1A2009084).
PY - 2014/9
Y1 - 2014/9
N2 - Here, we report the first genome sequence of a new virulent phage of Microbacterium oxydans, termed vB_MoxS-ISF9, which was isolated from sewage. Transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family Siphoviridae. The vB_MoxS-ISF9 DNA was completely sequenced and found to be 59,254 bp in length, with a G+C content of 62.76% and 120 putative open reading frames (ORFs). The predicted protein products of the ORFs were identified, and their sequences were analyzed. In a comparison with all available phage genomes, vB_MoxS-ISF9 did not show any significant similarity to other previously reported bacteriophages. To the beast of our knowledge, this is the first report of the isolation and complete genomic sequencing of a virulent phage against a member of the genus Microbacterium.
AB - Here, we report the first genome sequence of a new virulent phage of Microbacterium oxydans, termed vB_MoxS-ISF9, which was isolated from sewage. Transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family Siphoviridae. The vB_MoxS-ISF9 DNA was completely sequenced and found to be 59,254 bp in length, with a G+C content of 62.76% and 120 putative open reading frames (ORFs). The predicted protein products of the ORFs were identified, and their sequences were analyzed. In a comparison with all available phage genomes, vB_MoxS-ISF9 did not show any significant similarity to other previously reported bacteriophages. To the beast of our knowledge, this is the first report of the isolation and complete genomic sequencing of a virulent phage against a member of the genus Microbacterium.
UR - http://www.scopus.com/inward/record.url?scp=84907879699&partnerID=8YFLogxK
U2 - 10.1007/s00705-014-2092-5
DO - 10.1007/s00705-014-2092-5
M3 - Article
C2 - 24777828
AN - SCOPUS:84907879699
VL - 159
SP - 2537
EP - 2540
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 9
ER -