Complete Genome Sequence of Paenibacillus sp. CAA11

A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing

Gyeongtaek Gong, Hyun Ju Oh, Sukhyeong Cho, Seil Kim, Min-Kyu Oh, Youngsoon Um, Sun Mi Lee

Research output: Contribution to journalArticle

Abstract

Several bioprocessing technologies, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP), have been highlighted to produce bio-based fuels and chemicals from lignocellulosic biomass. Successful CBP, an efficient and economical lignocellulosic biorefinery process compared with other processes, requires microorganisms with sufficient cellulolytic activity and biofuel/chemical-producing ability. Here, we report the complete genome of Paenibacillus sp. CAA11, a newly isolated promising microbial host for CBP-producing ethanol and organic acids from cellulose. The genome of Paenibacillus sp. CAA11 comprises one 4,888,410 bp chromosome with a G + C content of 48.68% containing 4418 protein-coding genes, 102 tRNA genes, and 39 rRNA genes. The functionally active cellulase, encoded by CAA_GH5 was identified to belong to glycosyl hydrolase family 5 (GH5) and consisted of a catalytic domain and a cellulose-binding domain 3 (CBM3). When cellulolytic activity of CAA_GH5 was assayed through Congo red method by measuring the size of halo zone, the recombinant Bacillus subtilis RIK1285 expressing CAA_GH5 showed a comparable cellulolytic activity to B. subtilis RIK1285 expressing Cel5, a previously verified powerful bacterial cellulase. This study demonstrates the potential of Paenibacillus sp. CAA11 as a CBP-enabling microbe for cost-effective biofuels/chemicals production from lignocellulosic biomass.

Original languageEnglish
Pages (from-to)732-737
Number of pages6
JournalCurrent Microbiology
Volume76
Issue number6
DOIs
Publication statusPublished - 2019 Jun 15

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Paenibacillus
Hydrolases
Genome
Biofuels
Cellulase
Bacillus subtilis
Cellulose
Biomass
Fermentation
Congo Red
Base Composition
Transfer RNA
rRNA Genes
Catalytic Domain
Hydrolysis
Ethanol
Chromosomes
Technology
Costs and Cost Analysis
Acids

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Complete Genome Sequence of Paenibacillus sp. CAA11 : A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing. / Gong, Gyeongtaek; Oh, Hyun Ju; Cho, Sukhyeong; Kim, Seil; Oh, Min-Kyu; Um, Youngsoon; Lee, Sun Mi.

In: Current Microbiology, Vol. 76, No. 6, 15.06.2019, p. 732-737.

Research output: Contribution to journalArticle

Gong, Gyeongtaek ; Oh, Hyun Ju ; Cho, Sukhyeong ; Kim, Seil ; Oh, Min-Kyu ; Um, Youngsoon ; Lee, Sun Mi. / Complete Genome Sequence of Paenibacillus sp. CAA11 : A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing. In: Current Microbiology. 2019 ; Vol. 76, No. 6. pp. 732-737.
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