Complete genome sequencing of Shigella sp. PAMC 28760: Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation

So Ra Han, Do Wan Kim, Byeollee Kim, Young Min Chi, Seunghyun Kang, Hyun Park, Sang Hee Jung, Jun Hyuck Lee, Tae Jin Oh

Research output: Contribution to journalArticle

Abstract

Shigella sp. PAMC 28760 (isolated from Himantormia sp. lichen in Antarctica) is a gram-negative, non-sporulating bacterium that has cellulolytic and amylolytic characteristics as well as glycogen metabolic pathways. In this study, we isolated S. sp. PAMC 28760 from Antarctic lichen, and present the complete genome sequence with annotations describing its unique features. The genome sequence has 58.85% GC content, 4,278 coding DNA sequences, 85 tRNAs, and 22 rRNA operons. 16S rRNA gene sequence analyses revealed strain PAMC 28760 as a potentially new species of genus Shigella, showing various differences from pathogenic bacteria reported previously. dbCAN2 analyses revealed 91 genes related to carbohydrate-metabolizing enzymes. S. sp. PAMC 28760 likely degrades polysaccharide starch to obtain glucose for energy conservation. This study provides a foundation for understanding Shigella survival adaptation mechanisms under extremely cold Antarctic conditions.

Original languageEnglish
Article number103759
JournalMicrobial Pathogenesis
Volume137
DOIs
Publication statusPublished - 2019 Dec

Fingerprint

Shigella
Glycogen
Lichens
Genome
rRNA Operon
Genes
Bacteria
Base Composition
Transfer RNA
Metabolic Networks and Pathways
rRNA Genes
Starch
Polysaccharides
Sequence Analysis
Carbohydrates
Glucose
Enzymes

Keywords

  • Amylolytic characteristic
  • Antarctic lichen
  • CAZyme
  • Genome sequencing
  • Shigella

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Complete genome sequencing of Shigella sp. PAMC 28760 : Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation. / Han, So Ra; Kim, Do Wan; Kim, Byeollee; Chi, Young Min; Kang, Seunghyun; Park, Hyun; Jung, Sang Hee; Lee, Jun Hyuck; Oh, Tae Jin.

In: Microbial Pathogenesis, Vol. 137, 103759, 12.2019.

Research output: Contribution to journalArticle

Han, So Ra ; Kim, Do Wan ; Kim, Byeollee ; Chi, Young Min ; Kang, Seunghyun ; Park, Hyun ; Jung, Sang Hee ; Lee, Jun Hyuck ; Oh, Tae Jin. / Complete genome sequencing of Shigella sp. PAMC 28760 : Identification of CAZyme genes and analysis of their potential role in glycogen metabolism for cold survival adaptation. In: Microbial Pathogenesis. 2019 ; Vol. 137.
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