Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes

M. T. Le, H. Choi, M. K. Choi, D. T. Nguyen, J. H. Kim, H. G. Seo, S. Y. Cha, K. Seo, T. Chun, L. B. Schook, C. Park

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We previously reported the development of genomic-DNA-based high-resolution genotyping methods for SLA-DQB1 and DRB1. Here, we report the successful typing of SLA-DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA-DQA using intronic sequence information of SLA-DQA exon 2 that we had obtained from 12 animals with different SLA-DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA-DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.

Original languageEnglish
Pages (from-to)528-535
Number of pages8
JournalTissue Antigens
Volume80
Issue number6
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • Direct sequencing
  • Haplotype
  • Major histocompatibility complex
  • Polymerase chain reaction
  • SLA-DQA
  • Swine
  • Swine leukocyte antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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    Le, M. T., Choi, H., Choi, M. K., Nguyen, D. T., Kim, J. H., Seo, H. G., Cha, S. Y., Seo, K., Chun, T., Schook, L. B., & Park, C. (2012). Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes. Tissue Antigens, 80(6), 528-535. https://doi.org/10.1111/tan.12017