Comprehensive DNA barcodes for species identification and discovery of cryptic diversity in mayfly larvae from South Korea: Implications for freshwater ecosystem biomonitoring

Kyong In Suh, Jeong Mi Hwang, Yeon Jae Bae, Ji Hyoun Kang

Research output: Contribution to journalArticle

Abstract

DNA barcoding of aquatic macroinvertebrates holds much promise as a tool for taxonomic research and for providing baseline reference for phylogenetic analysis and aquatic ecosystem biomonitoring. We obtained 112 novel sequences of the barcode region of the mitochondrial DNA cytochrome c oxidase subunit I gene representing 11 families, 25 genera, and 43 species of mayfly (Insecta: Ephemeroptera) from South Korea. No species shared barcode sequences and all can be identified with barcodes with a possible exception of some species. Minimum levels of interspecific genetic distances ranged from 6.7 to 32.9% (mean: 23.7%), whereas average levels of intraspecific divergence was 3.7%. The latter value was inflated by the presence of very high divergences within some taxa. In fact, approximately 33.3% (15/45) of the species included two or more haplotype clusters showing greater than 5.0% sequence divergence and some values were as high as 32.9%. Many of the species with high intraspecific divergences are para- or polyphyletic and represent the possibility of species complexes. Our study suggests that type or topotype specimens should be sequenced to identify accurate barcoding clusters with morphological species concepts and also to determine the status of currently synonymized species.

Original languageEnglish
JournalEntomological Research
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

barcoding
DNA barcoding
South Korea
Ephemeroptera
larvae
aquatic invertebrates
Insecta
cytochrome-c oxidase
genetic distance
haplotypes
mitochondrial DNA
species identification
freshwater ecosystems
phylogeny
genes

Keywords

  • DNA barcode
  • Ephemeroptera
  • Korea
  • larvae
  • mayfly

ASJC Scopus subject areas

  • Insect Science

Cite this

@article{235e47370bcd4029bc43ed6361353620,
title = "Comprehensive DNA barcodes for species identification and discovery of cryptic diversity in mayfly larvae from South Korea: Implications for freshwater ecosystem biomonitoring",
abstract = "DNA barcoding of aquatic macroinvertebrates holds much promise as a tool for taxonomic research and for providing baseline reference for phylogenetic analysis and aquatic ecosystem biomonitoring. We obtained 112 novel sequences of the barcode region of the mitochondrial DNA cytochrome c oxidase subunit I gene representing 11 families, 25 genera, and 43 species of mayfly (Insecta: Ephemeroptera) from South Korea. No species shared barcode sequences and all can be identified with barcodes with a possible exception of some species. Minimum levels of interspecific genetic distances ranged from 6.7 to 32.9{\%} (mean: 23.7{\%}), whereas average levels of intraspecific divergence was 3.7{\%}. The latter value was inflated by the presence of very high divergences within some taxa. In fact, approximately 33.3{\%} (15/45) of the species included two or more haplotype clusters showing greater than 5.0{\%} sequence divergence and some values were as high as 32.9{\%}. Many of the species with high intraspecific divergences are para- or polyphyletic and represent the possibility of species complexes. Our study suggests that type or topotype specimens should be sequenced to identify accurate barcoding clusters with morphological species concepts and also to determine the status of currently synonymized species.",
keywords = "DNA barcode, Ephemeroptera, Korea, larvae, mayfly",
author = "Suh, {Kyong In} and Hwang, {Jeong Mi} and Bae, {Yeon Jae} and Kang, {Ji Hyoun}",
year = "2019",
month = "1",
day = "1",
doi = "10.1111/1748-5967.12334",
language = "English",
journal = "Entomological Research",
issn = "1748-5967",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Comprehensive DNA barcodes for species identification and discovery of cryptic diversity in mayfly larvae from South Korea

T2 - Implications for freshwater ecosystem biomonitoring

AU - Suh, Kyong In

AU - Hwang, Jeong Mi

AU - Bae, Yeon Jae

AU - Kang, Ji Hyoun

PY - 2019/1/1

Y1 - 2019/1/1

N2 - DNA barcoding of aquatic macroinvertebrates holds much promise as a tool for taxonomic research and for providing baseline reference for phylogenetic analysis and aquatic ecosystem biomonitoring. We obtained 112 novel sequences of the barcode region of the mitochondrial DNA cytochrome c oxidase subunit I gene representing 11 families, 25 genera, and 43 species of mayfly (Insecta: Ephemeroptera) from South Korea. No species shared barcode sequences and all can be identified with barcodes with a possible exception of some species. Minimum levels of interspecific genetic distances ranged from 6.7 to 32.9% (mean: 23.7%), whereas average levels of intraspecific divergence was 3.7%. The latter value was inflated by the presence of very high divergences within some taxa. In fact, approximately 33.3% (15/45) of the species included two or more haplotype clusters showing greater than 5.0% sequence divergence and some values were as high as 32.9%. Many of the species with high intraspecific divergences are para- or polyphyletic and represent the possibility of species complexes. Our study suggests that type or topotype specimens should be sequenced to identify accurate barcoding clusters with morphological species concepts and also to determine the status of currently synonymized species.

AB - DNA barcoding of aquatic macroinvertebrates holds much promise as a tool for taxonomic research and for providing baseline reference for phylogenetic analysis and aquatic ecosystem biomonitoring. We obtained 112 novel sequences of the barcode region of the mitochondrial DNA cytochrome c oxidase subunit I gene representing 11 families, 25 genera, and 43 species of mayfly (Insecta: Ephemeroptera) from South Korea. No species shared barcode sequences and all can be identified with barcodes with a possible exception of some species. Minimum levels of interspecific genetic distances ranged from 6.7 to 32.9% (mean: 23.7%), whereas average levels of intraspecific divergence was 3.7%. The latter value was inflated by the presence of very high divergences within some taxa. In fact, approximately 33.3% (15/45) of the species included two or more haplotype clusters showing greater than 5.0% sequence divergence and some values were as high as 32.9%. Many of the species with high intraspecific divergences are para- or polyphyletic and represent the possibility of species complexes. Our study suggests that type or topotype specimens should be sequenced to identify accurate barcoding clusters with morphological species concepts and also to determine the status of currently synonymized species.

KW - DNA barcode

KW - Ephemeroptera

KW - Korea

KW - larvae

KW - mayfly

UR - http://www.scopus.com/inward/record.url?scp=85059641677&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059641677&partnerID=8YFLogxK

U2 - 10.1111/1748-5967.12334

DO - 10.1111/1748-5967.12334

M3 - Article

AN - SCOPUS:85059641677

JO - Entomological Research

JF - Entomological Research

SN - 1748-5967

ER -