Concentration of arginine and optimal time of hypertonic saline in restoration of T-cell dysfunction

Sungwoo Moon, Sung Hyuk Choi, Han Jin Cho, Young Hoon Yun, Jung-Youn Kim, Yun Sik Hong, Todd Costantini, Vishal Bansal

Research output: Contribution to journalArticle

Abstract

Background: Hypertonic saline (HS) restores prostaglandin E2 (PGE2)-induced T-cell suppression in the presence of 1100 μM arginine. However, under arginine-free culture conditions, HS dose not restore T-cell proliferation. Therefore, we wanted to determine if HS can restore PGE2-induced T-cell suppression in the presence of 80 μM of arginine, the physiologically relevant arginine concentration. We also wanted to determine the concentration of arginine that induces HS restoration of PGE 2-suppressed T-cell proliferation and whether HS restoration of T-cell dysfunction is dependent on the injection time of HS. Materials and Methods: Jurkat cells were cultured in media containing 0, 40, 80, 400, 800, or 1100 μM arginine. In both the PGE2-stimulated and HS-treated group, we measured cell proliferation using MTT assay and arginase activity. We also measured cell proliferation relative to HS injection time. Results: In 80 μM arginine, HS did not restore Jurkat cell proliferation that had been suppressed by PGE2. Increased concentrations of arginine in the media increased MTT cell proliferation. In 800 μM arginine media, HS restored PGE2-suppressed Jurkat cell proliferation to normal. HS restored PGE2-suppressed Jurkat cell proliferation when it was added at 2 h, similar to at same time and 1 h after PGE2 stimulation. Conclusions: In order to restore PGE2-suppressed Jurkat cell proliferation, HS requires at least 800 μM arginine. HS restored PGE2-suppressed Jurkat cell proliferation even though HS was added at 2 h after PGE2 stimulation.

Original languageEnglish
JournalJournal of Surgical Research
Volume163
Issue number1
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Dinoprostone
Arginine
Cell Proliferation
Jurkat Cells
T-Lymphocytes
Arginase
Injections
Prostaglandins E

Keywords

  • arginine
  • hypertonic saline
  • T-cells
  • time
  • trauma

ASJC Scopus subject areas

  • Surgery

Cite this

Concentration of arginine and optimal time of hypertonic saline in restoration of T-cell dysfunction. / Moon, Sungwoo; Choi, Sung Hyuk; Cho, Han Jin; Yun, Young Hoon; Kim, Jung-Youn; Hong, Yun Sik; Costantini, Todd; Bansal, Vishal.

In: Journal of Surgical Research, Vol. 163, No. 1, 01.09.2010.

Research output: Contribution to journalArticle

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abstract = "Background: Hypertonic saline (HS) restores prostaglandin E2 (PGE2)-induced T-cell suppression in the presence of 1100 μM arginine. However, under arginine-free culture conditions, HS dose not restore T-cell proliferation. Therefore, we wanted to determine if HS can restore PGE2-induced T-cell suppression in the presence of 80 μM of arginine, the physiologically relevant arginine concentration. We also wanted to determine the concentration of arginine that induces HS restoration of PGE 2-suppressed T-cell proliferation and whether HS restoration of T-cell dysfunction is dependent on the injection time of HS. Materials and Methods: Jurkat cells were cultured in media containing 0, 40, 80, 400, 800, or 1100 μM arginine. In both the PGE2-stimulated and HS-treated group, we measured cell proliferation using MTT assay and arginase activity. We also measured cell proliferation relative to HS injection time. Results: In 80 μM arginine, HS did not restore Jurkat cell proliferation that had been suppressed by PGE2. Increased concentrations of arginine in the media increased MTT cell proliferation. In 800 μM arginine media, HS restored PGE2-suppressed Jurkat cell proliferation to normal. HS restored PGE2-suppressed Jurkat cell proliferation when it was added at 2 h, similar to at same time and 1 h after PGE2 stimulation. Conclusions: In order to restore PGE2-suppressed Jurkat cell proliferation, HS requires at least 800 μM arginine. HS restored PGE2-suppressed Jurkat cell proliferation even though HS was added at 2 h after PGE2 stimulation.",
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AU - Moon, Sungwoo

AU - Choi, Sung Hyuk

AU - Cho, Han Jin

AU - Yun, Young Hoon

AU - Kim, Jung-Youn

AU - Hong, Yun Sik

AU - Costantini, Todd

AU - Bansal, Vishal

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N2 - Background: Hypertonic saline (HS) restores prostaglandin E2 (PGE2)-induced T-cell suppression in the presence of 1100 μM arginine. However, under arginine-free culture conditions, HS dose not restore T-cell proliferation. Therefore, we wanted to determine if HS can restore PGE2-induced T-cell suppression in the presence of 80 μM of arginine, the physiologically relevant arginine concentration. We also wanted to determine the concentration of arginine that induces HS restoration of PGE 2-suppressed T-cell proliferation and whether HS restoration of T-cell dysfunction is dependent on the injection time of HS. Materials and Methods: Jurkat cells were cultured in media containing 0, 40, 80, 400, 800, or 1100 μM arginine. In both the PGE2-stimulated and HS-treated group, we measured cell proliferation using MTT assay and arginase activity. We also measured cell proliferation relative to HS injection time. Results: In 80 μM arginine, HS did not restore Jurkat cell proliferation that had been suppressed by PGE2. Increased concentrations of arginine in the media increased MTT cell proliferation. In 800 μM arginine media, HS restored PGE2-suppressed Jurkat cell proliferation to normal. HS restored PGE2-suppressed Jurkat cell proliferation when it was added at 2 h, similar to at same time and 1 h after PGE2 stimulation. Conclusions: In order to restore PGE2-suppressed Jurkat cell proliferation, HS requires at least 800 μM arginine. HS restored PGE2-suppressed Jurkat cell proliferation even though HS was added at 2 h after PGE2 stimulation.

AB - Background: Hypertonic saline (HS) restores prostaglandin E2 (PGE2)-induced T-cell suppression in the presence of 1100 μM arginine. However, under arginine-free culture conditions, HS dose not restore T-cell proliferation. Therefore, we wanted to determine if HS can restore PGE2-induced T-cell suppression in the presence of 80 μM of arginine, the physiologically relevant arginine concentration. We also wanted to determine the concentration of arginine that induces HS restoration of PGE 2-suppressed T-cell proliferation and whether HS restoration of T-cell dysfunction is dependent on the injection time of HS. Materials and Methods: Jurkat cells were cultured in media containing 0, 40, 80, 400, 800, or 1100 μM arginine. In both the PGE2-stimulated and HS-treated group, we measured cell proliferation using MTT assay and arginase activity. We also measured cell proliferation relative to HS injection time. Results: In 80 μM arginine, HS did not restore Jurkat cell proliferation that had been suppressed by PGE2. Increased concentrations of arginine in the media increased MTT cell proliferation. In 800 μM arginine media, HS restored PGE2-suppressed Jurkat cell proliferation to normal. HS restored PGE2-suppressed Jurkat cell proliferation when it was added at 2 h, similar to at same time and 1 h after PGE2 stimulation. Conclusions: In order to restore PGE2-suppressed Jurkat cell proliferation, HS requires at least 800 μM arginine. HS restored PGE2-suppressed Jurkat cell proliferation even though HS was added at 2 h after PGE2 stimulation.

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