Conditioned media of ASC-17D Sertoli cells induce G1-growth arrest of DU145 human prostate cancer cells

Hyeog Kang, Sang Wook Kang, Sang-Hyun Choi, Kwang Ho Lee, Boe Gwun Chun, Bon Hong Min

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We studied the effects of ASC-17D rat Sertoli cell-conditioned media (rSCCM) on the proliferation of the DU145 prostate cancer cells. rSCCM was prepared from ASC-17D cells cultured in DMEM/F-12 serum-free media at a nonpermissive temperature of 40°C, which is the condition for the high expression of clusterin. We found that rSCCM could inhibit the proliferation of DU145 cells by arresting the cell cycle in the G1 phase in a dose-dependent manner. This growth arresting activity was abolished by boiling rSCCM for 5 min. The G1 growth-inhibiting activity of rSCCM was also detected in other prostate-originated cancer cells examined (i.e., LNCaP and PC-3) but not in other cells (ASC-17D, HepG2, SK-N-SH, and NIH3T3). Western blot analysis of partially purified growth inhibiting fractions with the clusterin antibody showed that the cytostatic factor in rSCCM was not clusterin. This cytostatic factor was semipurified by DEAE-Sepharose, ammonium sulfate precipitation, and Phenyl-Sepharose column chromatography, and was estimated to have a molecular weight of 88 kDa by Sephacryl S-300 gel filtration.

Original languageEnglish
Pages (from-to)508-514
Number of pages7
JournalJournal of Biochemistry and Molecular Biology
Volume31
Issue number5
Publication statusPublished - 1998 Sep 30

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Sertoli Cells
Conditioned Culture Medium
Rats
Prostatic Neoplasms
Cells
Clusterin
Proto-Oncogene Proteins c-mos
Growth
Agarose Chromatography
Column chromatography
Serum-Free Culture Media
Ammonium Sulfate
G1 Phase
Sepharose
Boiling liquids
Gel Chromatography
Cultured Cells
Cell Cycle
Molecular Weight
Western Blotting

Keywords

  • Conditioned media
  • DU145 prostate cancer cell
  • Growth arrest
  • Sertoli cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Conditioned media of ASC-17D Sertoli cells induce G1-growth arrest of DU145 human prostate cancer cells. / Kang, Hyeog; Kang, Sang Wook; Choi, Sang-Hyun; Lee, Kwang Ho; Chun, Boe Gwun; Min, Bon Hong.

In: Journal of Biochemistry and Molecular Biology, Vol. 31, No. 5, 30.09.1998, p. 508-514.

Research output: Contribution to journalArticle

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