Abstract
We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.
| Original language | English |
|---|---|
| Pages (from-to) | 2074-2076 |
| Number of pages | 3 |
| Journal | Optics Letters |
| Volume | 33 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - 2008 Sep 15 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Confocal diffraction phase microscopy of live cells. / Lue, Niyom; Choi, Wonshik; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.; Popescu, Gabriel.
In: Optics Letters, Vol. 33, No. 18, 15.09.2008, p. 2074-2076.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Confocal diffraction phase microscopy of live cells
AU - Lue, Niyom
AU - Choi, Wonshik
AU - Badizadegan, Kamran
AU - Dasari, Ramachandra R.
AU - Feld, Michael S.
AU - Popescu, Gabriel
PY - 2008/9/15
Y1 - 2008/9/15
N2 - We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.
AB - We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.
UR - http://www.scopus.com/inward/record.url?scp=54749150661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54749150661&partnerID=8YFLogxK
U2 - 10.1364/OL.33.002074
DO - 10.1364/OL.33.002074
M3 - Article
VL - 33
SP - 2074
EP - 2076
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 18
ER -