Confocal diffraction phase microscopy of live cells

Niyom Lue, Wonshik Choi, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld, Gabriel Popescu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.

Original languageEnglish
Pages (from-to)2074-2076
Number of pages3
JournalOptics Letters
Volume33
Issue number18
DOIs
Publication statusPublished - 2008 Sep 15
Externally publishedYes

Fingerprint

Confocal Microscopy
Microscopy
Interferometry
microscopy
HT29 Cells
Polystyrenes
cells
Microspheres
diffraction
Erythrocytes
Cell Membrane
erythrocytes
polystyrene
interferometry
sensitivity
geometry

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Lue, N., Choi, W., Badizadegan, K., Dasari, R. R., Feld, M. S., & Popescu, G. (2008). Confocal diffraction phase microscopy of live cells. Optics Letters, 33(18), 2074-2076. https://doi.org/10.1364/OL.33.002074

Confocal diffraction phase microscopy of live cells. / Lue, Niyom; Choi, Wonshik; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.; Popescu, Gabriel.

In: Optics Letters, Vol. 33, No. 18, 15.09.2008, p. 2074-2076.

Research output: Contribution to journalArticle

Lue, N, Choi, W, Badizadegan, K, Dasari, RR, Feld, MS & Popescu, G 2008, 'Confocal diffraction phase microscopy of live cells', Optics Letters, vol. 33, no. 18, pp. 2074-2076. https://doi.org/10.1364/OL.33.002074
Lue N, Choi W, Badizadegan K, Dasari RR, Feld MS, Popescu G. Confocal diffraction phase microscopy of live cells. Optics Letters. 2008 Sep 15;33(18):2074-2076. https://doi.org/10.1364/OL.33.002074
Lue, Niyom ; Choi, Wonshik ; Badizadegan, Kamran ; Dasari, Ramachandra R. ; Feld, Michael S. ; Popescu, Gabriel. / Confocal diffraction phase microscopy of live cells. In: Optics Letters. 2008 ; Vol. 33, No. 18. pp. 2074-2076.
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