Confocal diffraction phase microscopy of live cells

Niyom Lue; Wonshik Choi; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld; Gabriel Popescu

doi:10.1364/OL.33.002074

Confocal diffraction phase microscopy of live cells

Niyom Lue, Wonshik Choi, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld, Gabriel Popescu

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.

Original language	English
Pages (from-to)	2074-2076
Number of pages	3
Journal	Optics Letters
Volume	33
Issue number	18
DOIs	https://doi.org/10.1364/OL.33.002074
Publication status	Published - 2008 Sep 15
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.33.002074

Cite this

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abstract = "We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.",

author = "Niyom Lue and Wonshik Choi and Kamran Badizadegan and Dasari, {Ramachandra R.} and Feld, {Michael S.} and Gabriel Popescu",

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AU - Lue, Niyom

AU - Choi, Wonshik

AU - Badizadegan, Kamran

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

AU - Popescu, Gabriel

PY - 2008/9/15

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AB - We present a new quantitative phase microscopy technique, confocal diffraction phase microscopy, which provides quantitative phase measurements from localized sites on a sample with high sensitivity. The technique combines common-path interferometry with confocal microscopy in a transmission geometry. The capability of the technique for static imaging is demonstrated by imaging polystyrene microspheres and live HT29 cells, while dynamic imaging is demonstrated by quantifying the nanometer scale fluctuations of red blood cell membranes.

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M3 - Article

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JO - Optics Letters

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Confocal diffraction phase microscopy of live cells

Abstract

ASJC Scopus subject areas

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