Confocal imaging of biological tissues using second harmonic generation

Beop Min Kim, Patrick Stoller, Karen Reiser, Jurgen Eichler, Ming Yan, Alexander Rubenchik, Luiz Da Silva

Research output: Contribution to journalConference article

13 Citations (Scopus)

Abstract

A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3914
Publication statusPublished - 2000
Externally publishedYes
EventLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical - San Jose, CA, USA
Duration: 2000 Jan 222000 Jan 27

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Kim, B. M., Stoller, P., Reiser, K., Eichler, J., Yan, M., Rubenchik, A., & Da Silva, L. (2000). Confocal imaging of biological tissues using second harmonic generation. Proceedings of SPIE - The International Society for Optical Engineering, 3914, 435-440.