Confocal imaging of biological tissues using second harmonic generation

Beop-Min Kim, Patrick Stoller, Karen Reiser, Jurgen Eichler, Ming Yan, Alexander Rubenchik, Luiz Da Silva

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages435-440
Number of pages6
Volume3914
Publication statusPublished - 2000
Externally publishedYes
EventLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical - San Jose, CA, USA
Duration: 2000 Jan 222000 Jan 27

Other

OtherLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
CitySan Jose, CA, USA
Period00/1/2200/1/27

Fingerprint

Harmonic generation
harmonic generations
Tissue
Imaging techniques
collagens
Collagen
Imaging systems
harmonics
wound healing
chickens
Confocal microscopy
blood vessels
Blood vessels
abnormalities
teeth
progressions
Skin
microscopy
magnetic permeability

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kim, B-M., Stoller, P., Reiser, K., Eichler, J., Yan, M., Rubenchik, A., & Da Silva, L. (2000). Confocal imaging of biological tissues using second harmonic generation. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3914, pp. 435-440). Society of Photo-Optical Instrumentation Engineers.

Confocal imaging of biological tissues using second harmonic generation. / Kim, Beop-Min; Stoller, Patrick; Reiser, Karen; Eichler, Jurgen; Yan, Ming; Rubenchik, Alexander; Da Silva, Luiz.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3914 Society of Photo-Optical Instrumentation Engineers, 2000. p. 435-440.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kim, B-M, Stoller, P, Reiser, K, Eichler, J, Yan, M, Rubenchik, A & Da Silva, L 2000, Confocal imaging of biological tissues using second harmonic generation. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3914, Society of Photo-Optical Instrumentation Engineers, pp. 435-440, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, San Jose, CA, USA, 00/1/22.
Kim B-M, Stoller P, Reiser K, Eichler J, Yan M, Rubenchik A et al. Confocal imaging of biological tissues using second harmonic generation. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3914. Society of Photo-Optical Instrumentation Engineers. 2000. p. 435-440
Kim, Beop-Min ; Stoller, Patrick ; Reiser, Karen ; Eichler, Jurgen ; Yan, Ming ; Rubenchik, Alexander ; Da Silva, Luiz. / Confocal imaging of biological tissues using second harmonic generation. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3914 Society of Photo-Optical Instrumentation Engineers, 2000. pp. 435-440
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