Conformational recovery and preservation of protein nature from heat-induced denaturation by water-soluble phospholipid polymer conjugation

Ji Hun Seo, Ryosuke Matsuno, Yan Lee, Madoka Takai, Kazuhiko Ishihara

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16 Citations (Scopus)

Abstract

The effect of water-soluble phospholipid polymer conjugated to a protein on conformational change during heat-quenched stress was investigated in this study. Well-defined pyridine disulfide end-functional poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was synthesized by well controlled atom transfer radical polymerization method. Synthesized PMPC was the site specifically conjugated to a protein, and the effect on conformational change during heat-quenched stress was estimated by circular dichroism and fluorescence study. As a result, a single phospholipid polymer chain conjugated to a protein give a great effect on conformational preservation in both secondary and tertiary structures even after the heat-quenced process. Moreover, even the conformational recovery which gave the completely reversible conformational preservation was observed in circular dichroism study. The resulting protein activity was also confirmed, and no significant decline was induced by heat-quenched stress.

Original languageEnglish
Pages (from-to)4859-4867
Number of pages9
JournalBiomaterials
Volume30
Issue number28
DOIs
Publication statusPublished - 2009 Oct 1
Externally publishedYes

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Keywords

  • Bioconjugation
  • Conformation change
  • Enzyme
  • Phospholipid polymer
  • Protein

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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