Conjugated polyelectrolyte and aptamer based potassium assay via single- and two-step fluorescence energy transfer with a tunable dynamic detection range

Bao Lam Nguyen, Ji Eun Jeong, In Hwan Jung, Boram Kim, Van Sang Le, Inhong Kim, Kwangseuk Kyhm, Han Young Woo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A new potassium ion detection assay was developed using a dye-labeled aptamer and conjugated polyelectrolyte (CPE) as a signaling platform via 1-step and 2-step fluorescence resonance energy transfer. Guanine-rich K +-specific aptamers were designed as K+ ion recognition species with 6-carboxyfluorescein (6-FAM) and 6-carboxytetramethylrhodamine (6-TAMRA) at both termini. In the presence of K+ ions, the aptamers undergo a conformational change from an unfolded to folded form by forming a G-quadruplex with K+, bringing two dyes in proximity. FRET-induced 6-TAMRA emission was proportional to [K+] in a range of 22.5 μm-100 mm in water without interference by the presence of excess Na + ions (100 mm). Upon the addition of CPE, a two-step FRET process from CPE to 6-TAMRA via 6-FAM was enabled, showing an intensified 6-TAMRA signal with K+ ions. The dynamic detection range and limit of detection (LOD) was fine-tuned from ∼millimolar to ∼nanomolar concentrations of K+ by modulating the signal amplification effect of CPE. The LOD was determined to be ≈3.0 nm. This detection assay also showed high selectivity against other metal ions. This sensing scheme can be extended to the detection of a wide range of target materials by simply modifying the recognition aptamer sequence. A conformational change in the K+-specific aptamer to form a G-quadruplex produces a clear turn on and off signal in the presence or absence of K+ ions. Highly sensitive and selective K+ detection is observed via single- and two-step FRET processes with a tunable sensing range and limit of detection.

Original languageEnglish
Pages (from-to)1748-1757
Number of pages10
JournalAdvanced Functional Materials
Volume24
Issue number12
DOIs
Publication statusPublished - 2014 Mar 26

Keywords

  • FRET
  • aptamers
  • conjugated polyelectrolytes
  • fluorescence
  • potassium

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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