Consecutive intercalation of a cationic porphyrin dimmer between the GC base-pairs: A kinetic study

Lindan Gong, Changyun Lee, Gyeongwon Kim, Young Sun Lee, Suk Joong Lee, Seog K. Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

One of the porphyrin moieties of the porphyrin dimer intercalates rapidly between the DNA base-pairs, followed by the slow intercalation of the other. The slow intercalation of the second porphyrin moiety of porphyrin dimer to poly[d(G-C)2] was investigated by normal absorption and circular dichroism spectroscopy. The change in absorbance in the Soret band of porphyrin upon association with poly[d(G-C)2] can be best elucidated by the combination of the two single exponential curves, suggesting the intercalation occurred via two independent first order reactions. Activation energies of these two first order reactions were calculated to be 0.37 kcal/mol and 3.19 kcal/mol, respectively. The intercalation associated with lower activation energy can be assigned to the intercalation of second porphyrin moiety to 5′CG3′ site while that with higher activation energy to 5′GC3′ intercalation site. Increasing the flexibility of poly[d(G-C)2] by adding Na+ ion resulted in an enhancement of the reaction rate for both steps in an exponential manner.

Original languageEnglish
Pages (from-to)1303-1307
Number of pages5
JournalJournal of Porphyrins and Phthalocyanines
Volume16
Issue number12
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • DNA
  • activation energy
  • bis-TMPyP
  • intercalation

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Consecutive intercalation of a cationic porphyrin dimmer between the GC base-pairs: A kinetic study'. Together they form a unique fingerprint.

Cite this