Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici

Sujon Sarowar, Young Jin Kim, Eui Nam Kim, Ki Deok Kim, Jun Young Choi, Nam In Hyung, Jeong Sheop Shin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The potential role of two pathogen-induced pepper genes, encoding basic pathogenesis-related protein 1 (CABPR1) and ascorbate peroxidase-like 1 (CAPOA1), in tolerance against phytopathogens was examined in transgenic tomato (Lycopersicum esculentum cv. House Momotaro) plants. Polymerase chain reaction and reverse transcription-polymerase chain reaction analyses using gene-specific primers revealed that the pepper CABPR1 and CAPOA1 genes were integrated into the tomato genome. The constitutive expression of CABPR1 and CAPOA1 in the tomato did not exhibit any morphological abnormalities. However, these transgenic tomato plants showed enhanced tolerance to the oomycete pathogen Phytophthora capsici, and very weak resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. These results suggest that overexpression of CABPR1 and CAPOA1 in tomato plants altered their resistance responses to pathogenic attack.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalPlant Cell, Tissue and Organ Culture
Volume86
Issue number1
DOIs
Publication statusPublished - 2006 Jul 1

Fingerprint

Oomycetes
Phytophthora
Phytophthora capsici
Lycopersicon esculentum
Ascorbate Peroxidases
ascorbate peroxidase
pathogenesis
tomatoes
pathogens
Genes
genes
pepper
house plants
genetically modified organisms
Pseudomonas syringae pv. tomato
pathogenesis-related proteins
Pseudomonas syringae
Polymerase Chain Reaction
plant pathogens
Genetically Modified Plants

Keywords

  • CABPR1 - Capsicum annuum basic pathogenesis-related protein 1
  • CAPOA1 - Capsicum annuum ascorbate peroxidase-like 1
  • Disease resistance
  • Phytophthora capsici
  • Pseudomonas syringae pv. tomato
  • Transgenic plant

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici. / Sarowar, Sujon; Kim, Young Jin; Kim, Eui Nam; Kim, Ki Deok; Choi, Jun Young; Hyung, Nam In; Shin, Jeong Sheop.

In: Plant Cell, Tissue and Organ Culture, Vol. 86, No. 1, 01.07.2006, p. 7-14.

Research output: Contribution to journalArticle

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