Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici

Sujon Sarowar; Young Jin Kim; Eui Nam Kim; Ki Deok Kim; Jun Young Choi; Nam In Hyung; Jeong Sheop Shin

doi:10.1007/s11240-006-9090-6

Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici

Sujon Sarowar, Young Jin Kim, Eui Nam Kim, Ki Deok Kim, Jun Young Choi, Nam In Hyung, Jeong Sheop Shin

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The potential role of two pathogen-induced pepper genes, encoding basic pathogenesis-related protein 1 (CABPR1) and ascorbate peroxidase-like 1 (CAPOA1), in tolerance against phytopathogens was examined in transgenic tomato (Lycopersicum esculentum cv. House Momotaro) plants. Polymerase chain reaction and reverse transcription-polymerase chain reaction analyses using gene-specific primers revealed that the pepper CABPR1 and CAPOA1 genes were integrated into the tomato genome. The constitutive expression of CABPR1 and CAPOA1 in the tomato did not exhibit any morphological abnormalities. However, these transgenic tomato plants showed enhanced tolerance to the oomycete pathogen Phytophthora capsici, and very weak resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. These results suggest that overexpression of CABPR1 and CAPOA1 in tomato plants altered their resistance responses to pathogenic attack.

Original language	English
Pages (from-to)	7-14
Number of pages	8
Journal	Plant Cell, Tissue and Organ Culture
Volume	86
Issue number	1
DOIs	https://doi.org/10.1007/s11240-006-9090-6
Publication status	Published - 2006 Jul

Keywords

CABPR1 - Capsicum annuum basic pathogenesis-related protein 1
CAPOA1 - Capsicum annuum ascorbate peroxidase-like 1
Disease resistance
Phytophthora capsici
Pseudomonas syringae pv. tomato
Transgenic plant

ASJC Scopus subject areas

Horticulture

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10.1007/s11240-006-9090-6

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@article{10eb380c68334183ab7b62b39840a515,

title = "Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici",

abstract = "The potential role of two pathogen-induced pepper genes, encoding basic pathogenesis-related protein 1 (CABPR1) and ascorbate peroxidase-like 1 (CAPOA1), in tolerance against phytopathogens was examined in transgenic tomato (Lycopersicum esculentum cv. House Momotaro) plants. Polymerase chain reaction and reverse transcription-polymerase chain reaction analyses using gene-specific primers revealed that the pepper CABPR1 and CAPOA1 genes were integrated into the tomato genome. The constitutive expression of CABPR1 and CAPOA1 in the tomato did not exhibit any morphological abnormalities. However, these transgenic tomato plants showed enhanced tolerance to the oomycete pathogen Phytophthora capsici, and very weak resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. These results suggest that overexpression of CABPR1 and CAPOA1 in tomato plants altered their resistance responses to pathogenic attack.",

keywords = "CABPR1 - Capsicum annuum basic pathogenesis-related protein 1, CAPOA1 - Capsicum annuum ascorbate peroxidase-like 1, Disease resistance, Phytophthora capsici, Pseudomonas syringae pv. tomato, Transgenic plant",

author = "Sujon Sarowar and Kim, {Young Jin} and Kim, {Eui Nam} and Kim, {Ki Deok} and Choi, {Jun Young} and Hyung, {Nam In} and Shin, {Jeong Sheop}",

note = "Funding Information: This study was supported by the special grant from the Agricultural R&D Promotion Center funded by the Ministry of Agriculture and Forestry.",

year = "2006",

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doi = "10.1007/s11240-006-9090-6",

language = "English",

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AU - Sarowar, Sujon

AU - Kim, Young Jin

AU - Kim, Eui Nam

AU - Kim, Ki Deok

AU - Choi, Jun Young

AU - Hyung, Nam In

AU - Shin, Jeong Sheop

N1 - Funding Information: This study was supported by the special grant from the Agricultural R&D Promotion Center funded by the Ministry of Agriculture and Forestry.

PY - 2006/7

Y1 - 2006/7

N2 - The potential role of two pathogen-induced pepper genes, encoding basic pathogenesis-related protein 1 (CABPR1) and ascorbate peroxidase-like 1 (CAPOA1), in tolerance against phytopathogens was examined in transgenic tomato (Lycopersicum esculentum cv. House Momotaro) plants. Polymerase chain reaction and reverse transcription-polymerase chain reaction analyses using gene-specific primers revealed that the pepper CABPR1 and CAPOA1 genes were integrated into the tomato genome. The constitutive expression of CABPR1 and CAPOA1 in the tomato did not exhibit any morphological abnormalities. However, these transgenic tomato plants showed enhanced tolerance to the oomycete pathogen Phytophthora capsici, and very weak resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. These results suggest that overexpression of CABPR1 and CAPOA1 in tomato plants altered their resistance responses to pathogenic attack.

AB - The potential role of two pathogen-induced pepper genes, encoding basic pathogenesis-related protein 1 (CABPR1) and ascorbate peroxidase-like 1 (CAPOA1), in tolerance against phytopathogens was examined in transgenic tomato (Lycopersicum esculentum cv. House Momotaro) plants. Polymerase chain reaction and reverse transcription-polymerase chain reaction analyses using gene-specific primers revealed that the pepper CABPR1 and CAPOA1 genes were integrated into the tomato genome. The constitutive expression of CABPR1 and CAPOA1 in the tomato did not exhibit any morphological abnormalities. However, these transgenic tomato plants showed enhanced tolerance to the oomycete pathogen Phytophthora capsici, and very weak resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. These results suggest that overexpression of CABPR1 and CAPOA1 in tomato plants altered their resistance responses to pathogenic attack.

KW - CABPR1 - Capsicum annuum basic pathogenesis-related protein 1

KW - CAPOA1 - Capsicum annuum ascorbate peroxidase-like 1

KW - Disease resistance

KW - Phytophthora capsici

KW - Pseudomonas syringae pv. tomato

KW - Transgenic plant

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M3 - Article

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SN - 0167-6857

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Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici

Abstract

Keywords

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