Construction of a functional network for common DNA damage responses in Escherichia coli

Jungeui Hong, Joo Myung Ahn, Byoung Chan Kim, Man Bock Gu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, we aim to identify a common, general mode of toxic action in Escherichia coli when experiencing DNA damage, irrespective of the agents used. We conducted or collected 69 microarray data from seven different DNA damaging agents. In a quantitative manner, we constructed a probable DNA damage stress network, entitled the 'Functional Linked Network (FLN)', which consists of 399 significantly perturbed genes and the 1283 interactions among them. The SOS response related genes (LexA modules) were found to be dominantly activated by DNA damage, irrespective of the agents. Several minor, plausible modules were also implicated in this network, and appear to be related with the metabolic inhibition response to DNA damage or mediate the induction of SOS response. This systems and comparison approach across a variety of genotoxic agents may serve as a starting point to specify some of the unknown and common features of DNA damage responses in bacteria.

Original languageEnglish
Pages (from-to)514-524
Number of pages11
JournalGenomics
Volume93
Issue number6
DOIs
Publication statusPublished - 2009 Jun 1

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DNA Damage
Escherichia coli
Toxic Actions
Gene Regulatory Networks
Bacteria
DNA
Genes

Keywords

  • DNA damage response
  • Escherichia coli
  • Functional network
  • Quantitative index system

ASJC Scopus subject areas

  • Genetics

Cite this

Construction of a functional network for common DNA damage responses in Escherichia coli. / Hong, Jungeui; Ahn, Joo Myung; Kim, Byoung Chan; Gu, Man Bock.

In: Genomics, Vol. 93, No. 6, 01.06.2009, p. 514-524.

Research output: Contribution to journalArticle

Hong, Jungeui ; Ahn, Joo Myung ; Kim, Byoung Chan ; Gu, Man Bock. / Construction of a functional network for common DNA damage responses in Escherichia coli. In: Genomics. 2009 ; Vol. 93, No. 6. pp. 514-524.
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