Construction of a nrdA: :luxCDABE fusion and its use in Escherichia coli as a DNA damage biosensor

Ee Taek Hwang, Joo Myung Ahn, Byoung Chan Kim, Man Bock Gu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The promoter of nrdA gene which is related with DNA synthesis was used to construct a DNA damage sensitive biosensor. A recombinant bioluminescent E. coli strain, BBTNrdA, harboring a plasmid with the nrdA promoter fused to the luxCDABE operon, was successfully constructed. Its response to various chemicals including genotoxic chemicals substantiates it as a DNA damage biosensor. In characterization, three different classes of toxicants were used: DNA damaging chemicals, oxidative stress chemicals, and phenolics. BBTNrdA only responded strongly to DNA damaging chemicals, such as nalidixic acid (NDA), mitomycin C (MMC), 1-methyl-1-nitroso-N-methylguanidine (MNNG), and 4-nitroquinoline N-oxide (4-NQO). In contrast, there were no responses from the oxidative stress chemicals and phenolics, except from hydrogen peroxide (H2O 2) which is known to cause DNA damage indirectly. Therefore, the results of the study demonstrate that BBTNrdA can be used as a DNA damage biosensor.

Original languageEnglish
Pages (from-to)1297-1307
Number of pages11
JournalSensors
Volume8
Issue number2
Publication statusPublished - 2008 Feb 1

Fingerprint

Biosensing Techniques
Escherichia
bioinstrumentation
Biosensors
Escherichia coli
DNA Damage
DNA
Fusion reactions
deoxyribonucleic acid
fusion
damage
Oxidative Stress
Methylguanidine
4-Nitroquinoline-1-oxide
Oxidative stress
Nalidixic Acid
Mitomycin
Operon
Hydrogen Peroxide
Plasmids

Keywords

  • Bioluminescence bacteria
  • Biosensor
  • DNA damage response
  • Ribonucleoside diphosphate reductase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Construction of a nrdA : :luxCDABE fusion and its use in Escherichia coli as a DNA damage biosensor. / Hwang, Ee Taek; Ahn, Joo Myung; Kim, Byoung Chan; Gu, Man Bock.

In: Sensors, Vol. 8, No. 2, 01.02.2008, p. 1297-1307.

Research output: Contribution to journalArticle

Hwang, Ee Taek ; Ahn, Joo Myung ; Kim, Byoung Chan ; Gu, Man Bock. / Construction of a nrdA : :luxCDABE fusion and its use in Escherichia coli as a DNA damage biosensor. In: Sensors. 2008 ; Vol. 8, No. 2. pp. 1297-1307.
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