Construction of a nrdA::luxCDABE fusion and its use in Escherichia coli as a DNA damage biosensor

Ee Taek Hwang, Joo Myung Ahn, Byoung Chan Kim, Man Bock Gu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The promoter of nrdA gene which is related with DNA synthesis was used to construct a DNA damage sensitive biosensor. A recombinant bioluminescent E. coli strain, BBTNrdA, harboring a plasmid with the nrdA promoter fused to the luxCDABE operon, was successfully constructed. Its response to various chemicals including genotoxic chemicals substantiates it as a DNA damage biosensor. In characterization, three different classes of toxicants were used: DNA damaging chemicals, oxidative stress chemicals, and phenolics. BBTNrdA only responded strongly to DNA damaging chemicals, such as nalidixic acid (NDA), mitomycin C (MMC), 1-methyl-1-nitroso-N-methylguanidine (MNNG), and 4-nitroquinoline N-oxide (4-NQO). In contrast, there were no responses from the oxidative stress chemicals and phenolics, except from hydrogen peroxide (H2O 2) which is known to cause DNA damage indirectly. Therefore, the results of the study demonstrate that BBTNrdA can be used as a DNA damage biosensor.

Original languageEnglish
Pages (from-to)1297-1307
Number of pages11
JournalSensors
Volume8
Issue number2
DOIs
Publication statusPublished - 2008 Feb

Keywords

  • Bioluminescence bacteria
  • Biosensor
  • DNA damage response
  • Ribonucleoside diphosphate reductase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering

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