Construction of a reporter strain Pseudomonas putida for the detection of oxidative stress caused by environmental pollutants

Yunho Lee, Eunyoung Ahn, Sungsu Park, Eugene L. Madsen, Che Ok Jeon, Woojun Park

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A green fluorescent protein-based Pseudomonas putida reporter was successfully constructed and shown to be capable of detecting oxidative stress. In this whole-cell reporter, the promoter of the paraquat-inducible ferredoxin-NADP + reductase (fpr) was fused to a promoterless gfp gene on a broad-host-range promoter probe vector. Pseudomonas putida KT2440 harboring this reporter plasmid exhibited an increased level of gfp expression in the presence of redoxcycling agents (paraquat and menadione), hydrogen peroxide, and potential environmental pollutant chemicals such as toluene, paint thinner, gasoline, and diesel. Induction of fpr in the presence of these chemicals was confirmed using Northern blot analysis.

Original languageEnglish
Pages (from-to)386-390
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume16
Issue number3
Publication statusPublished - 2006 Mar 1

Fingerprint

Ferredoxin-NADP Reductase
Environmental Pollutants
Pseudomonas putida
Paraquat
Oxidative stress
Paint thinners
Oxidative Stress
Vitamin K 3
Gasoline
Paint
Host Specificity
Toluene
Green Fluorescent Proteins
Hydrogen peroxide
Northern Blotting
Hydrogen Peroxide
Plasmids
Genes
Proteins
Oxidoreductases

Keywords

  • Green fluorescence protein
  • Hydrogen peroxide
  • Menadione
  • Paint thinner
  • Paraquat

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Construction of a reporter strain Pseudomonas putida for the detection of oxidative stress caused by environmental pollutants. / Lee, Yunho; Ahn, Eunyoung; Park, Sungsu; Madsen, Eugene L.; Jeon, Che Ok; Park, Woojun.

In: Journal of Microbiology and Biotechnology, Vol. 16, No. 3, 01.03.2006, p. 386-390.

Research output: Contribution to journalArticle

Lee, Yunho ; Ahn, Eunyoung ; Park, Sungsu ; Madsen, Eugene L. ; Jeon, Che Ok ; Park, Woojun. / Construction of a reporter strain Pseudomonas putida for the detection of oxidative stress caused by environmental pollutants. In: Journal of Microbiology and Biotechnology. 2006 ; Vol. 16, No. 3. pp. 386-390.
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