Abstract
A green fluorescent protein-based Pseudomonas putida reporter was successfully constructed and shown to be capable of detecting oxidative stress. In this whole-cell reporter, the promoter of the paraquat-inducible ferredoxin-NADP + reductase (fpr) was fused to a promoterless gfp gene on a broad-host-range promoter probe vector. Pseudomonas putida KT2440 harboring this reporter plasmid exhibited an increased level of gfp expression in the presence of redoxcycling agents (paraquat and menadione), hydrogen peroxide, and potential environmental pollutant chemicals such as toluene, paint thinner, gasoline, and diesel. Induction of fpr in the presence of these chemicals was confirmed using Northern blot analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 386-390 |
| Number of pages | 5 |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 16 |
| Issue number | 3 |
| Publication status | Published - 2006 Mar 1 |
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Keywords
- Green fluorescence protein
- Hydrogen peroxide
- Menadione
- Paint thinner
- Paraquat
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Microbiology
- Bioengineering
Cite this
Construction of a reporter strain Pseudomonas putida for the detection of oxidative stress caused by environmental pollutants. / Lee, Yunho; Ahn, Eunyoung; Park, Sungsu; Madsen, Eugene L.; Jeon, Che Ok; Park, Woojun.
In: Journal of Microbiology and Biotechnology, Vol. 16, No. 3, 01.03.2006, p. 386-390.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Construction of a reporter strain Pseudomonas putida for the detection of oxidative stress caused by environmental pollutants
AU - Lee, Yunho
AU - Ahn, Eunyoung
AU - Park, Sungsu
AU - Madsen, Eugene L.
AU - Jeon, Che Ok
AU - Park, Woojun
PY - 2006/3/1
Y1 - 2006/3/1
N2 - A green fluorescent protein-based Pseudomonas putida reporter was successfully constructed and shown to be capable of detecting oxidative stress. In this whole-cell reporter, the promoter of the paraquat-inducible ferredoxin-NADP + reductase (fpr) was fused to a promoterless gfp gene on a broad-host-range promoter probe vector. Pseudomonas putida KT2440 harboring this reporter plasmid exhibited an increased level of gfp expression in the presence of redoxcycling agents (paraquat and menadione), hydrogen peroxide, and potential environmental pollutant chemicals such as toluene, paint thinner, gasoline, and diesel. Induction of fpr in the presence of these chemicals was confirmed using Northern blot analysis.
AB - A green fluorescent protein-based Pseudomonas putida reporter was successfully constructed and shown to be capable of detecting oxidative stress. In this whole-cell reporter, the promoter of the paraquat-inducible ferredoxin-NADP + reductase (fpr) was fused to a promoterless gfp gene on a broad-host-range promoter probe vector. Pseudomonas putida KT2440 harboring this reporter plasmid exhibited an increased level of gfp expression in the presence of redoxcycling agents (paraquat and menadione), hydrogen peroxide, and potential environmental pollutant chemicals such as toluene, paint thinner, gasoline, and diesel. Induction of fpr in the presence of these chemicals was confirmed using Northern blot analysis.
KW - Green fluorescence protein
KW - Hydrogen peroxide
KW - Menadione
KW - Paint thinner
KW - Paraquat
UR - http://www.scopus.com/inward/record.url?scp=33645548032&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645548032&partnerID=8YFLogxK
M3 - Article
VL - 16
SP - 386
EP - 390
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 3
ER -