Construction of a sodA: :luxCDABE fusion Escherichia coli: Comparison with a katG fusion strain through their responses to oxidative stresses

H. J. Lee, Man Bock Gu

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A recombinant bioluminescent Escherichia coli strain, EBHJ, (sodA::luxCDABE), containing the promoter for the manganese superoxide dismutase (sodA) gene fused to the Vibrio fischeri luxCDABE operon, was successfully constructed and characterized. Redox-cycling agents, such as paraquat and chromium, strongly induced a sodA- regulated response in dose-dependent manners, resulting in an increase of the bioluminescence. In a comparison with an existing oxidative stress responsive strain, DPD2511 (katG::luxCDABE), which is sensitive to H2O2, the mechanism of chemicals that cause oxidative damage was elucidated via the key transcriptional factors involved in induction of the sodA and katG promoters, i.e. SoxRS and OxyR, respectively. It was found that responses from the katG- and sodA-based strains were significantly different dependent upon the chemicals being tested. Therefore, EBHJ, alone or in parallel with DPD2511, can be used to characterize and monitor chemicals that cause oxidative damage.

Original languageEnglish
Pages (from-to)577-580
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume60
Issue number5
Publication statusPublished - 2003 Jan 1
Externally publishedYes

Fingerprint

Oxidative stress
Escherichia coli
Superoxide Dismutase
Oxidative Stress
Fusion reactions
Aliivibrio fischeri
Bioluminescence
Paraquat
Chromium
Operon
Oxidation-Reduction
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

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