TY - JOUR
T1 - Construction of a sodA::luxCDABE fusion Escherichia coli: Comparison with a katG fusion strain through their responses to oxidative stresses
AU - Lee, H. J.
AU - Gu, M. B.
N1 - Funding Information:
Acknowledgements This work was supported by the National Research Laboratory (2001 NRL) Program of Korea Institute of Science and Technology Evaluation and Planning (Project No. M10104000094–01J000004100). The authors thank Dr. Robert LaRossa for strain DPD2511.
PY - 2003/1
Y1 - 2003/1
N2 - A recombinant bioluminescent Escherichia coli strain, EBHJ, (sodA::luxCDABE), containing the promoter for the manganese superoxide dismutase (sodA) gene fused to the Vibrio fischeri luxCDABE operon, was successfully constructed and characterized. Redox-cycling agents, such as paraquat and chromium, strongly induced a sodA- regulated response in dose-dependent manners, resulting in an increase of the bioluminescence. In a comparison with an existing oxidative stress responsive strain, DPD2511 (katG::luxCDABE), which is sensitive to H2O2, the mechanism of chemicals that cause oxidative damage was elucidated via the key transcriptional factors involved in induction of the sodA and katG promoters, i.e. SoxRS and OxyR, respectively. It was found that responses from the katG- and sodA-based strains were significantly different dependent upon the chemicals being tested. Therefore, EBHJ, alone or in parallel with DPD2511, can be used to characterize and monitor chemicals that cause oxidative damage.
AB - A recombinant bioluminescent Escherichia coli strain, EBHJ, (sodA::luxCDABE), containing the promoter for the manganese superoxide dismutase (sodA) gene fused to the Vibrio fischeri luxCDABE operon, was successfully constructed and characterized. Redox-cycling agents, such as paraquat and chromium, strongly induced a sodA- regulated response in dose-dependent manners, resulting in an increase of the bioluminescence. In a comparison with an existing oxidative stress responsive strain, DPD2511 (katG::luxCDABE), which is sensitive to H2O2, the mechanism of chemicals that cause oxidative damage was elucidated via the key transcriptional factors involved in induction of the sodA and katG promoters, i.e. SoxRS and OxyR, respectively. It was found that responses from the katG- and sodA-based strains were significantly different dependent upon the chemicals being tested. Therefore, EBHJ, alone or in parallel with DPD2511, can be used to characterize and monitor chemicals that cause oxidative damage.
UR - http://www.scopus.com/inward/record.url?scp=0037255508&partnerID=8YFLogxK
U2 - 10.1007/s00253-002-1168-4
DO - 10.1007/s00253-002-1168-4
M3 - Article
C2 - 12536259
AN - SCOPUS:0037255508
SN - 0175-7598
VL - 60
SP - 577
EP - 580
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 5
ER -