Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser

Matthew A. Churgin, Liping He, John I. Murray, Christopher Fang-Yen

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The spatial and temporal control of transgene expression is an important tool in Caenorhabditis elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al., 2013) [1]. Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalMethods
Volume68
Issue number3
DOIs
Publication statusPublished - 2014 Aug 1

Keywords

  • C. elegans
  • Heat shock
  • Lasers
  • Transgenes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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