Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser

Matthew A. Churgin, Liping He, John I. Murray, Christopher Fang-Yen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The spatial and temporal control of transgene expression is an important tool in Caenorhabditis elegans biology. We previously described a method for evoking gene expression in arbitrary cells by using a focused pulsed infrared laser to induce a heat shock response (Churgin et al., 2013) [1]. Here we describe detailed methods for building and testing a system for performing single-cell heat shock. Steps include setting up the laser and associated components, coupling the laser beam to a microscope, and testing heat shock protocols. All steps can be carried out using readily available off-the-shelf components.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalMethods
Volume68
Issue number3
DOIs
Publication statusPublished - 2014 Aug 1

Fingerprint

Infrared lasers
Caenorhabditis elegans
Pulsed lasers
Transgenes
Lasers
Shock
Hot Temperature
Heat-Shock Response
Testing
Gene expression
Laser beams
Microscopes
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser. / Churgin, Matthew A.; He, Liping; Murray, John I.; Fang-Yen, Christopher.

In: Methods, Vol. 68, No. 3, 01.08.2014, p. 431-436.

Research output: Contribution to journalArticle

Churgin, Matthew A. ; He, Liping ; Murray, John I. ; Fang-Yen, Christopher. / Construction of a system for single-cell transgene induction in Caenorhabditis elegans using a pulsed infrared laser. In: Methods. 2014 ; Vol. 68, No. 3. pp. 431-436.
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