Construction of a vector generating both siRNA and a fluorescent reporter

A siRNA study in cultured neurons

Seung Yoon Yoon, Jung Eun Choi, Onyou Hwang, Hea Nam Hong, Heuiran Lee, Yoo Kyum Kim, Sung Woo Cho, Hyun Kim, Dong Hou Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

RNA interference is an important tool for gene silencing. However, its application to primary cultured cells has been limited by low transfection efficiencies. In this work we developed a vector which encodes both siRNA and red fluorescent protein. Using this vector we could markedly suppress green fluorescent protein (GFP) and bim an endogenous gene. Primary cultured cortical neurons transfected with siRNA against doublecortin showed that doublecortin expression was significantly inhibited in nearly all the transfected neurons. This vector identifies the transfected cells and should be useful for loss-of-gene function studies in neurons.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalMolecules and Cells
Volume18
Issue number1
Publication statusPublished - 2004 Aug 31

Fingerprint

Small Interfering RNA
Neurons
Gene Silencing
RNA Interference
Green Fluorescent Proteins
Genes
Transfection
Cultured Cells
red fluorescent protein

Keywords

  • Fluorescent reporter
  • Neuron
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Yoon, S. Y., Choi, J. E., Hwang, O., Hong, H. N., Lee, H., Kim, Y. K., ... Kim, D. H. (2004). Construction of a vector generating both siRNA and a fluorescent reporter: A siRNA study in cultured neurons. Molecules and Cells, 18(1), 127-130.

Construction of a vector generating both siRNA and a fluorescent reporter : A siRNA study in cultured neurons. / Yoon, Seung Yoon; Choi, Jung Eun; Hwang, Onyou; Hong, Hea Nam; Lee, Heuiran; Kim, Yoo Kyum; Cho, Sung Woo; Kim, Hyun; Kim, Dong Hou.

In: Molecules and Cells, Vol. 18, No. 1, 31.08.2004, p. 127-130.

Research output: Contribution to journalArticle

Yoon, SY, Choi, JE, Hwang, O, Hong, HN, Lee, H, Kim, YK, Cho, SW, Kim, H & Kim, DH 2004, 'Construction of a vector generating both siRNA and a fluorescent reporter: A siRNA study in cultured neurons', Molecules and Cells, vol. 18, no. 1, pp. 127-130.
Yoon SY, Choi JE, Hwang O, Hong HN, Lee H, Kim YK et al. Construction of a vector generating both siRNA and a fluorescent reporter: A siRNA study in cultured neurons. Molecules and Cells. 2004 Aug 31;18(1):127-130.
Yoon, Seung Yoon ; Choi, Jung Eun ; Hwang, Onyou ; Hong, Hea Nam ; Lee, Heuiran ; Kim, Yoo Kyum ; Cho, Sung Woo ; Kim, Hyun ; Kim, Dong Hou. / Construction of a vector generating both siRNA and a fluorescent reporter : A siRNA study in cultured neurons. In: Molecules and Cells. 2004 ; Vol. 18, No. 1. pp. 127-130.
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