Construction of hemispherical microwells using elastic PDMS membrane and application to embryoid bodies formation

Dae Ho Lee, Joong Yull Park, Eun Joong Lee, Yoon Young Choi, Beop-Min Kim, Sang Hoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The thin and highly elastic PDMS membrane was fabricated and the novel, easy and stable method to handle without rupture, folding and wrinkling. On basis of this novel technology, we have developed a method to construct the diverse 3D curved microstructures, whose size and shape are easily controllable. The fabricated concave structure was applied as the arrayed wells for the cell culture and uniformly sized murine embryoid bodies (EBs) were successfully formed. In addition, the diverse shaped microparticles with different materials were fabricated for the use of drug carrier and pH responsive micro-actuator element.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1195-1197
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

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ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Lee, D. H., Park, J. Y., Lee, E. J., Choi, Y. Y., Kim, B-M., & Lee, S. H. (2009). Construction of hemispherical microwells using elastic PDMS membrane and application to embryoid bodies formation. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1195-1197). Chemical and Biological Microsystems Society.