Construction of neurospheroids via surface modified concave microwells

Geon Hui Lee, Jaeho Lim, Ji Soo Park, Wonseok Lee, Dae Sung Yoon, Soo Hyun Kim, Myung Ki Kim, Sang Hoon Lee, Dong Hwee Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Developing a three-dimensional (3D) neural tissue model is important to comprehensively understand neural development and neuronal degeneration associated with various neurological disorders such as axonopathy and neuronopathy. Here, a new microplatform suitable for constructing neuronal spheroids (neurospheroids) was developed by modulating cell–surface interactions. The inner surface of a polydimethylsiloxane (PDMS) concave microwell array extensively used in in vitro cell aggregation was modified with typical extracellular matrix (ECM) molecules or carbon nanotubes to control neural spheroid formation. Modulating neuronal cell–ECM interactions could tune 3D intercellular interactions and spheroidal functionality. Neurite outgrowth, a neuronal marker for complex interneuronal signaling, was found to be tightly regulated by cell–ECM interactions in a confined space. Furthermore, amyloid-β (Aβ)-induced axonopathy representing a pathological feature of neurodegenerative diseases in vivo was examined in this study to monitor the degeneration of neurite outgrowth and alteration of neuronal morphology in these neurospheroids. The proposed neural tissue model could be used to study various neurodegenerative diseases in the future.

Original languageEnglish
Pages (from-to)341-351
Number of pages11
JournalJournal of Industrial and Engineering Chemistry
Volume62
DOIs
Publication statusPublished - 2018 Jun 25

Keywords

  • 3D neural tissues
  • Axonopathy
  • Cell–cell interactions
  • Cell–extracellular matrix interactions

ASJC Scopus subject areas

  • Chemical Engineering(all)

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