Construction of recombinant BCGs overexpressing antigen 85 complex and their protective efficacy against Mycobacterium tuberculosis infection in a mouse model

Seung Heon Lee, Bo Young Jeon, Young Gil Park, Hye Young Lee, Sang Nae Cho, Hyo Joon Kim, Gill Han Bai

Research output: Contribution to journalArticle

Abstract

Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed overexpression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- γ production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalTuberculosis and Respiratory Diseases
Volume57
Issue number2
DOIs
Publication statusPublished - 2004 Aug

Keywords

  • Recombinant BCG
  • Tuberculosis
  • Vaccine

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

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