Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein

Junghyun Hahn, Se Hoon Park, Jae Young Song, Soo Hwan An, Byung Yoon Ahn

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

To explore the swinepox virus (SPV) as a potential live vector for immunization, a vector was developed for the construction of a recombinant SPV carrying foreign genes. In this system, a foreign gene placed under the strong vaccinia virus promoter P11 can be inserted into the viral thymidine kinase (TK) gene, and the recombinant virus can be isolated in a non-selective medium by the co-expression of E. coli lacZ gene. Compared with the wild type virus, the TK-recombinant SPV showed a modest level of attenuation in porcine cells while more attenuation was observed in monkey or human cells. Using this system, a recombinant virus expressing the E2 glycoprotein of classical swine fever virus (CSFV) was produced. Engineered with the gX signal sequence of the pseudorabies virus, and transmembrane domain of E2, the E2 protein was expressed as a dimeric form in the cytoplasm of the infected cells.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalJournal of Virological Methods
Volume93
Issue number1-2
DOIs
Publication statusPublished - 2001

Keywords

  • CSFV
  • E2
  • Recombinant swinepox virus
  • Thymidine kinase

ASJC Scopus subject areas

  • Virology

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