Constructive remodeling of a synthetic endothelial extracellular matrix

Sewoon Han, Yoojin Shin, Hyo Eun Jeong, Jessie S. Jeon, Roger D. Kamm, Dongeun Huh, Lydia L. Sohn, Seok Chung

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally.

Original languageEnglish
Article number18290
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015 Dec 21

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Extracellular Matrix
Endothelial Cells
Vascular Endothelium
Nanofibers
Biological Models
Capillary Permeability
Tissue Engineering
Collagen Type I
Basement Membrane
Intercellular Signaling Peptides and Proteins
Phenotype
Research
Pharmaceutical Preparations

ASJC Scopus subject areas

  • General

Cite this

Constructive remodeling of a synthetic endothelial extracellular matrix. / Han, Sewoon; Shin, Yoojin; Jeong, Hyo Eun; Jeon, Jessie S.; Kamm, Roger D.; Huh, Dongeun; Sohn, Lydia L.; Chung, Seok.

In: Scientific Reports, Vol. 5, 18290, 21.12.2015.

Research output: Contribution to journalArticle

Han, S, Shin, Y, Jeong, HE, Jeon, JS, Kamm, RD, Huh, D, Sohn, LL & Chung, S 2015, 'Constructive remodeling of a synthetic endothelial extracellular matrix', Scientific Reports, vol. 5, 18290. https://doi.org/10.1038/srep18290
Han S, Shin Y, Jeong HE, Jeon JS, Kamm RD, Huh D et al. Constructive remodeling of a synthetic endothelial extracellular matrix. Scientific Reports. 2015 Dec 21;5. 18290. https://doi.org/10.1038/srep18290
Han, Sewoon ; Shin, Yoojin ; Jeong, Hyo Eun ; Jeon, Jessie S. ; Kamm, Roger D. ; Huh, Dongeun ; Sohn, Lydia L. ; Chung, Seok. / Constructive remodeling of a synthetic endothelial extracellular matrix. In: Scientific Reports. 2015 ; Vol. 5.
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