Constructive remodeling of a synthetic endothelial extracellular matrix

Sewoon Han, Yoojin Shin, Hyo Eun Jeong, Jessie S. Jeon, Roger D. Kamm, Dongeun Huh, Lydia L. Sohn, Seok Chung

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23 Citations (Scopus)

Abstract

The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally.

Original languageEnglish
Article number18290
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Dec 21

ASJC Scopus subject areas

  • General

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    Han, S., Shin, Y., Jeong, H. E., Jeon, J. S., Kamm, R. D., Huh, D., Sohn, L. L., & Chung, S. (2015). Constructive remodeling of a synthetic endothelial extracellular matrix. Scientific reports, 5, [18290]. https://doi.org/10.1038/srep18290