Continuous cultivation of a human intestinal microflora at high cell concentration via controlled culture recycle

Moo Heon Kang, Jeewon Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A bioreactor system equipped with a hollow fiber cross-filtration module was used for continuous cultivation of Lactobacillus acidophilus at high cell concentrations. The growth rate did not correlate with the lactate concentration if the residual glucose concentration was kept nearly zero in the culture broth. To achieve this, an effective control method of medium feed rate was developed on the basis of the correlation between the specific glucose consumption rate (v) and the specific cell growth rate (μ), i.e. v = 290μ + 0.39. Growth up to 50 g dry wt l-1 was achieved without glucose accumulation under the total cell recycle. Via the partial cell recycle, continuous biomass production was achieved with a steady-state L. acidophilus concentration and dilution rate being 40 gl-1 and 0.09 h-1.

Original languageEnglish
Pages (from-to)295-299
Number of pages5
JournalBiotechnology Letters
Volume20
Issue number3
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Glucose
Lactobacillus acidophilus
Growth
Cell growth
Bioreactors
Dilution
Lactic Acid
Biomass
Fibers
Gastrointestinal Microbiome

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Continuous cultivation of a human intestinal microflora at high cell concentration via controlled culture recycle. / Kang, Moo Heon; Lee, Jeewon.

In: Biotechnology Letters, Vol. 20, No. 3, 1998, p. 295-299.

Research output: Contribution to journalArticle

@article{fbf1bc99bbad4f318949fd1fa951e66b,
title = "Continuous cultivation of a human intestinal microflora at high cell concentration via controlled culture recycle",
abstract = "A bioreactor system equipped with a hollow fiber cross-filtration module was used for continuous cultivation of Lactobacillus acidophilus at high cell concentrations. The growth rate did not correlate with the lactate concentration if the residual glucose concentration was kept nearly zero in the culture broth. To achieve this, an effective control method of medium feed rate was developed on the basis of the correlation between the specific glucose consumption rate (v) and the specific cell growth rate (μ), i.e. v = 290μ + 0.39. Growth up to 50 g dry wt l-1 was achieved without glucose accumulation under the total cell recycle. Via the partial cell recycle, continuous biomass production was achieved with a steady-state L. acidophilus concentration and dilution rate being 40 gl-1 and 0.09 h-1.",
author = "Kang, {Moo Heon} and Jeewon Lee",
year = "1998",
doi = "10.1023/A:1005346406835",
language = "English",
volume = "20",
pages = "295--299",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Continuous cultivation of a human intestinal microflora at high cell concentration via controlled culture recycle

AU - Kang, Moo Heon

AU - Lee, Jeewon

PY - 1998

Y1 - 1998

N2 - A bioreactor system equipped with a hollow fiber cross-filtration module was used for continuous cultivation of Lactobacillus acidophilus at high cell concentrations. The growth rate did not correlate with the lactate concentration if the residual glucose concentration was kept nearly zero in the culture broth. To achieve this, an effective control method of medium feed rate was developed on the basis of the correlation between the specific glucose consumption rate (v) and the specific cell growth rate (μ), i.e. v = 290μ + 0.39. Growth up to 50 g dry wt l-1 was achieved without glucose accumulation under the total cell recycle. Via the partial cell recycle, continuous biomass production was achieved with a steady-state L. acidophilus concentration and dilution rate being 40 gl-1 and 0.09 h-1.

AB - A bioreactor system equipped with a hollow fiber cross-filtration module was used for continuous cultivation of Lactobacillus acidophilus at high cell concentrations. The growth rate did not correlate with the lactate concentration if the residual glucose concentration was kept nearly zero in the culture broth. To achieve this, an effective control method of medium feed rate was developed on the basis of the correlation between the specific glucose consumption rate (v) and the specific cell growth rate (μ), i.e. v = 290μ + 0.39. Growth up to 50 g dry wt l-1 was achieved without glucose accumulation under the total cell recycle. Via the partial cell recycle, continuous biomass production was achieved with a steady-state L. acidophilus concentration and dilution rate being 40 gl-1 and 0.09 h-1.

UR - http://www.scopus.com/inward/record.url?scp=0031941238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031941238&partnerID=8YFLogxK

U2 - 10.1023/A:1005346406835

DO - 10.1023/A:1005346406835

M3 - Article

AN - SCOPUS:0031941238

VL - 20

SP - 295

EP - 299

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 3

ER -