Continuous cultivation of a human intestinal microflora at high cell concentration via controlled culture recycle

Moo Heon Kang, Jeewon Lee

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A bioreactor system equipped with a hollow fiber cross-filtration module was used for continuous cultivation of Lactobacillus acidophilus at high cell concentrations. The growth rate did not correlate with the lactate concentration if the residual glucose concentration was kept nearly zero in the culture broth. To achieve this, an effective control method of medium feed rate was developed on the basis of the correlation between the specific glucose consumption rate (v) and the specific cell growth rate (μ), i.e. v = 290μ + 0.39. Growth up to 50 g dry wt l-1 was achieved without glucose accumulation under the total cell recycle. Via the partial cell recycle, continuous biomass production was achieved with a steady-state L. acidophilus concentration and dilution rate being 40 gl-1 and 0.09 h-1.

Original languageEnglish
Pages (from-to)295-299
Number of pages5
JournalBiotechnology letters
Volume20
Issue number3
DOIs
Publication statusPublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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