Continuous erythrocyte removal and leukocyte separation from whole blood based on viscoelastic cell focusing and the margination phenomenon

Jeonghun Nam, Jung Yoon, Jeeyong Kim, Woong Sik Jang, Chae Seung Lim

Research output: Contribution to journalArticle

Abstract

The removal of erythrocytes from whole blood is an essential step during sample preparations intended for biomedical analyses and clinical diagnoses. To address the limitations of present methods, such as centrifugation and chemical lysis, we propose a novel microfluidic device for erythrocyte removal with high-efficiency and leukocyte separation from bulk flows of highly concentrated erythrocytes using a viscoelastic non-Newtonian fluid. The proposed device is designed based on the principle of viscoelasticity-induced particle migration toward the center of the microchannel. In addition, we based the functionality of our device on a bio-inspired phenomenon known as margination according to which erythrocytes migrate to the axial center of blood vessels. Fluorescent particles (10 μm) were added to blood suspensions of various concentrations (hematocrit) of erythrocytes in viscoelastic polymer solutions. Optimal hematocrit and flow rate conditions were determined for erythrocyte removal and for the separation of 10 μm particles. We also demonstrated the capability of our device to separate leukocytes with high efficiency (˜94%) and with a high-enrichment factor (10-fold).

Original languageEnglish
JournalJournal of Chromatography A
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Leukocytes
Blood
Erythrocytes
Centrifugation
Viscoelasticity
Blood vessels
Lab-On-A-Chip Devices
Polymer solutions
Microchannels
Hematocrit
Microfluidics
Equipment and Supplies
Suspensions
Viscoelastic Substances
Flow rate
Device Removal
Fluids
Blood Vessels
Polymers

Keywords

  • Erythrocyte
  • High hematocrit
  • Leukocyte
  • Margination
  • Viscoelasticity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Continuous erythrocyte removal and leukocyte separation from whole blood based on viscoelastic cell focusing and the margination phenomenon. / Nam, Jeonghun; Yoon, Jung; Kim, Jeeyong; Jang, Woong Sik; Lim, Chae Seung.

In: Journal of Chromatography A, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Lim, Chae Seung

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N2 - The removal of erythrocytes from whole blood is an essential step during sample preparations intended for biomedical analyses and clinical diagnoses. To address the limitations of present methods, such as centrifugation and chemical lysis, we propose a novel microfluidic device for erythrocyte removal with high-efficiency and leukocyte separation from bulk flows of highly concentrated erythrocytes using a viscoelastic non-Newtonian fluid. The proposed device is designed based on the principle of viscoelasticity-induced particle migration toward the center of the microchannel. In addition, we based the functionality of our device on a bio-inspired phenomenon known as margination according to which erythrocytes migrate to the axial center of blood vessels. Fluorescent particles (10 μm) were added to blood suspensions of various concentrations (hematocrit) of erythrocytes in viscoelastic polymer solutions. Optimal hematocrit and flow rate conditions were determined for erythrocyte removal and for the separation of 10 μm particles. We also demonstrated the capability of our device to separate leukocytes with high efficiency (˜94%) and with a high-enrichment factor (10-fold).

AB - The removal of erythrocytes from whole blood is an essential step during sample preparations intended for biomedical analyses and clinical diagnoses. To address the limitations of present methods, such as centrifugation and chemical lysis, we propose a novel microfluidic device for erythrocyte removal with high-efficiency and leukocyte separation from bulk flows of highly concentrated erythrocytes using a viscoelastic non-Newtonian fluid. The proposed device is designed based on the principle of viscoelasticity-induced particle migration toward the center of the microchannel. In addition, we based the functionality of our device on a bio-inspired phenomenon known as margination according to which erythrocytes migrate to the axial center of blood vessels. Fluorescent particles (10 μm) were added to blood suspensions of various concentrations (hematocrit) of erythrocytes in viscoelastic polymer solutions. Optimal hematocrit and flow rate conditions were determined for erythrocyte removal and for the separation of 10 μm particles. We also demonstrated the capability of our device to separate leukocytes with high efficiency (˜94%) and with a high-enrichment factor (10-fold).

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