Continuous fabrication of biocatalyst immobilized microparticles using photopolymerization and immiscible liquids in microfluidic systems

Won Je Jeong, Jeong Yun Kim, Jaebum Choo, Eun Kyu Lee, Chang Soo Han, David J. Beebe, Gi Hun Seong, Sang Hoon Lee

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

We report a novel technique for manufacturing polymeric microparticles containing biocatalysts by the behavior of immiscible liquids in microfluidic systems and in situ photopolymerization. The approach utilizes a UV-polymerizable hydrogel/enzyme solution and an immiscible oil solution. The oil and hydrogel solutions form emulsions in pressure-driven flow in microchannels at high values of the dimensionless capillary number (Ca). The resultant hydrogel droplets are then polymerized in situ via exposure to 365 nm UV light. This technique allows for the generation of monodisperse particles whose size can be controlled by the regulation of flow rates. In addition, both manufacturing microparticles and immobilizing biocatalysts can be performed simultaneously and continuously.

Original languageEnglish
Pages (from-to)3738-3741
Number of pages4
JournalLangmuir
Volume21
Issue number9
DOIs
Publication statusPublished - 2005 Apr 26

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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