Continuous inertial microparticle and blood cell separation in straight channels with local microstructures

Zhenlong Wu, Yu Chen, Moran Wang, Aram Chung

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Fluid inertia which has conventionally been neglected in microfluidics has been gaining much attention for particle and cell manipulation because inertia-based methods inherently provide simple, passive, precise and high-throughput characteristics. Particularly, the inertial approach has been applied to blood separation for various biomedical research studies mainly using spiral microchannels. For higher throughput, parallelization is essential; however, it is difficult to realize using spiral channels because of their large two dimensional layouts. In this work, we present a novel inertial platform for continuous sheathless particle and blood cell separation in straight microchannels containing microstructures. Microstructures within straight channels exert secondary flows to manipulate particle positions similar to Dean flow in curved channels but with higher controllability. Through a balance between inertial lift force and microstructure-induced secondary flow, we deterministically position microspheres and cells based on their sizes to be separated downstream. Using our inertial platform, we successfully sorted microparticles and fractionized blood cells with high separation efficiencies, high purities and high throughputs. The inertial separation platform developed here can be operated to process diluted blood with a throughput of 10.8 mL min -1 via radially arrayed single channels with one inlet and two rings of outlets.

Original languageEnglish
Pages (from-to)532-542
Number of pages11
JournalLab on a Chip
Volume16
Issue number3
DOIs
Publication statusPublished - 2016 Feb 7
Externally publishedYes

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Cell Separation
Blood Cells
Blood
Cells
Throughput
Microstructure
Microfluidics
Secondary flow
Microchannels
Microspheres
Biomedical Research
Controllability
Fluids

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Continuous inertial microparticle and blood cell separation in straight channels with local microstructures. / Wu, Zhenlong; Chen, Yu; Wang, Moran; Chung, Aram.

In: Lab on a Chip, Vol. 16, No. 3, 07.02.2016, p. 532-542.

Research output: Contribution to journalArticle

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