Continuous supply of glucose and glycerol enhances biotransformation of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid in recombinant Escherichia coli

Yong Han Cho, Soo Jung Kim, Hyun Woo Kim, Ji Young Kim, Jun Seok Gwak, Donghwa Chung, Kyoung Heon Kim, Kyungmoon Park, Yong Cheol Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus luteus and a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440. Feeding of glucose or glycerol facilitated both the preparation of high-density cell biocatalyst and supply of the NAD+ and NADPH cofactors. By the glucose feeding strategy, 30.8 g/L of the engineered E. coli cells produced 29.7 mM of 11-HOUA with 1.9 mM/h of productivity, which were 1.8 and 1.6 times higher than the same biotransformation without the glucose feeding, respectively. Intermittent addition of glycerol increased 11-HOUA productivity by 16% compared to that by the glucose feeding. Finally, 34.5 mM of 11-HOUA concentration, 77% conversion and 2.2 mM/h productivity were obtained using 31.6 g/L of cell biocatalyst along with the glycerol addition. It was concluded that supplementation of additional carbon sources in biotransformation process would be a potent strategy to increase the performance of fatty acid conversion.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalJournal of Biotechnology
Volume253
DOIs
Publication statusPublished - 2017 Jul 10

Keywords

  • (E)-11-(Heptanoyloxy) undec-9-enoic acid
  • Alcohol dehydrogenase
  • Baeyer-Villiger monooxygenase
  • Fed-batch
  • Recombinant Escherichia coli
  • Ricinoleic acid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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