Abstract
This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus luteus and a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440. Feeding of glucose or glycerol facilitated both the preparation of high-density cell biocatalyst and supply of the NAD+ and NADPH cofactors. By the glucose feeding strategy, 30.8 g/L of the engineered E. coli cells produced 29.7 mM of 11-HOUA with 1.9 mM/h of productivity, which were 1.8 and 1.6 times higher than the same biotransformation without the glucose feeding, respectively. Intermittent addition of glycerol increased 11-HOUA productivity by 16% compared to that by the glucose feeding. Finally, 34.5 mM of 11-HOUA concentration, 77% conversion and 2.2 mM/h productivity were obtained using 31.6 g/L of cell biocatalyst along with the glycerol addition. It was concluded that supplementation of additional carbon sources in biotransformation process would be a potent strategy to increase the performance of fatty acid conversion.
| Original language | English |
|---|---|
| Pages (from-to) | 34-39 |
| Number of pages | 6 |
| Journal | Journal of Biotechnology |
| Volume | 253 |
| DOIs | |
| Publication status | Published - 2017 Jul 10 |
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Keywords
- (E)-11-(Heptanoyloxy) undec-9-enoic acid
- Alcohol dehydrogenase
- Baeyer-Villiger monooxygenase
- Fed-batch
- Recombinant Escherichia coli
- Ricinoleic acid
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Continuous supply of glucose and glycerol enhances biotransformation of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid in recombinant Escherichia coli. / Cho, Yong Han; Kim, Soo Jung; Kim, Hyun Woo; Kim, Ji Young; Gwak, Jun Seok; Chung, Donghwa; Kim, Kyoung Heon; Park, Kyungmoon; Park, Yong Cheol.
In: Journal of Biotechnology, Vol. 253, 10.07.2017, p. 34-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Continuous supply of glucose and glycerol enhances biotransformation of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid in recombinant Escherichia coli
AU - Cho, Yong Han
AU - Kim, Soo Jung
AU - Kim, Hyun Woo
AU - Kim, Ji Young
AU - Gwak, Jun Seok
AU - Chung, Donghwa
AU - Kim, Kyoung Heon
AU - Park, Kyungmoon
AU - Park, Yong Cheol
PY - 2017/7/10
Y1 - 2017/7/10
N2 - This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus luteus and a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440. Feeding of glucose or glycerol facilitated both the preparation of high-density cell biocatalyst and supply of the NAD+ and NADPH cofactors. By the glucose feeding strategy, 30.8 g/L of the engineered E. coli cells produced 29.7 mM of 11-HOUA with 1.9 mM/h of productivity, which were 1.8 and 1.6 times higher than the same biotransformation without the glucose feeding, respectively. Intermittent addition of glycerol increased 11-HOUA productivity by 16% compared to that by the glucose feeding. Finally, 34.5 mM of 11-HOUA concentration, 77% conversion and 2.2 mM/h productivity were obtained using 31.6 g/L of cell biocatalyst along with the glycerol addition. It was concluded that supplementation of additional carbon sources in biotransformation process would be a potent strategy to increase the performance of fatty acid conversion.
AB - This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus luteus and a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440. Feeding of glucose or glycerol facilitated both the preparation of high-density cell biocatalyst and supply of the NAD+ and NADPH cofactors. By the glucose feeding strategy, 30.8 g/L of the engineered E. coli cells produced 29.7 mM of 11-HOUA with 1.9 mM/h of productivity, which were 1.8 and 1.6 times higher than the same biotransformation without the glucose feeding, respectively. Intermittent addition of glycerol increased 11-HOUA productivity by 16% compared to that by the glucose feeding. Finally, 34.5 mM of 11-HOUA concentration, 77% conversion and 2.2 mM/h productivity were obtained using 31.6 g/L of cell biocatalyst along with the glycerol addition. It was concluded that supplementation of additional carbon sources in biotransformation process would be a potent strategy to increase the performance of fatty acid conversion.
KW - (E)-11-(Heptanoyloxy) undec-9-enoic acid
KW - Alcohol dehydrogenase
KW - Baeyer-Villiger monooxygenase
KW - Fed-batch
KW - Recombinant Escherichia coli
KW - Ricinoleic acid
UR - http://www.scopus.com/inward/record.url?scp=85019677825&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019677825&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2017.05.014
DO - 10.1016/j.jbiotec.2017.05.014
M3 - Article
C2 - 28536060
AN - SCOPUS:85019677825
VL - 253
SP - 34
EP - 39
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
ER -