Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells

Sun Jong Kim, Seon Hye Cheon, Seung Jun Yoo, Jinie Kwon, Jong Hyuk Park, Chul Geun Kim, Kunsoo Rhee, Seungkwon You, Joo Yong Lee, Sung Il Roh, Hyun Soo Yoon

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Although basic fibroblast growth factor (FGF2) is generally included in the media for maintenance of human embryonic stem cells (hESCs), the action of FGF2 in these cells has not been well defined. Here, we determined the roles of FGF2 in maintaining hESC self-renewal. Withdrawal of FGF2 from the media led to acquisition of typical differentiated characteristics in hESCs. In the presence of FGF2, which is normally required for proliferation in an undifferentiated state, inhibition of phosphatidylinositol 3-kinase (PI3K)/Akt/PKB signal stimulated differentiation and attenuated the expression of extracellular matrix (ECM) molecules. We suggest that FGF2 maintains hESC self-renewal by supporting stable expression of ECM molecules through activation of the PI3K/Akt/PKB pathway.

Original languageEnglish
Pages (from-to)534-540
Number of pages7
JournalFEBS Letters
Volume579
Issue number2
DOIs
Publication statusPublished - 2005 Jan 17

Keywords

  • Differentiation and undifferentiation
  • Extracellular matrix molecule
  • Fibroblast growth factor signaling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Kim, S. J., Cheon, S. H., Yoo, S. J., Kwon, J., Park, J. H., Kim, C. G., Rhee, K., You, S., Lee, J. Y., Roh, S. I., & Yoon, H. S. (2005). Contribution of the PI3K/Akt/PKB signal pathway to maintenance of self-renewal in human embryonic stem cells. FEBS Letters, 579(2), 534-540. https://doi.org/10.1016/j.febslet.2004.12.024