TY - JOUR
T1 - Contributions of enhanced endogenous microbial metabolism via inoculation with a novel microbial consortium into an anoxic side-stream reactor to in-situ sludge reduction for landfill leachate treatment
AU - Cheng, Yu
AU - Chon, Kangmin
AU - Ren, Xianghao
AU - Lee, Ying
AU - Kou, Yingying
AU - Wu, Yan
AU - Shen, Mingyu
AU - Hwang, Moon Hyun
AU - Chae, Kyu Jung
N1 - Funding Information:
This work was funded by the Open Research Fund Program of the Key Laboratory of Urban Stormwater System and Water Environment ( Beijing University of Civil Engineering and Architecture ) and partially supported by the Korea Environment Industry & Technology Institute ( KEITI ) through the Aquatic Ecosystem Conservation Research Program, funded by the Korea Ministry of Environment (MOE) ( RE202001312 ).
Funding Information:
Fig. 5b and figure S1 shows the relative abundance of bacterial communities in SR-B and SR-C at the class and genus level. A total of 19 different classes and 28 different genera were discovered in the two samples (abundance in at least one sample > 0.1% found in the Ribosomal Database Project). Alphaproteobacteria (SR-B = 11.0%, SR-C = 3.9%) and Betaproteobacteria (SR-B = 44.3%, SR-C = 69.3%), as the dominant classes, had similar regulation found in the side-stream sludge reduction system (OSA), and it was suggested to induce cell lysis and release intracellular substances in oligotrophic environments (Cheng et al., 2017, 2018). Gammaproteobacteria (SR-B = 8.9%, SR-C = 1.3%) related to EPS secretion easily adhered to the surface of carriers (Cheng et al., 2017). Bacteroidia (SR-B = 5.8%, SR-C = 0.1%) assume the responsibility of the hydrolysis and fermentation of organic matter (e.g., proteins and polysaccharides) (Cheng et al., 2018). Moreover, Sphingobacteria (SR-B = 11.4%, SR-C = 8.9%) are associated with the secretion of extracellular enzymes and hydrolysis fermentation, which may be beneficial to the degradation of EPS (An et al., 2020). Besides, the Acinetobacter (SR-B = 2.45%, SR-C = 0%) cooperating with the EPS producers (Thauera: SR-B = 40.7%, SR-C = 65.6%) were new species abundant in SR-B (Peng et al., 2021). Ottowia (SR-B = 1.8%, SR-C = 0.5%) as a functionally important hydrolysis bacteria plays a significant role in organics degradation, which may participate in the degradation and conversion of EPS (Li et al., 2020b). Thermomonas (SR-B = 3.9%, SR-C = 0.2%) as denitrifiers may contribute to the process of endogenous denitrification and the secretion of protein-like matters (Wang et al., 2018; Wu et al., 2019). Hence, abundant bacteria related to the conversion and degradation of EPS was enriched in SR-B, and these observations support the assumption that the differences in the microbial community composition play a critical role in in-situ sludge reduction (Wang et al., 2019).This work was funded by the Open Research Fund Program of the Key Laboratory of Urban Stormwater System and Water Environment (Beijing University of Civil Engineering and Architecture) and partially supported by the Korea Environment Industry & Technology Institute (KEITI) through the Aquatic Ecosystem Conservation Research Program, funded by the Korea Ministry of Environment (MOE) (RE202001312).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10/1
Y1 - 2021/10/1
N2 - In-situ sludge reduction plays a significant role in reducing excess sludge production. This study investigated the role of beneficial microorganisms (BM) in the anoxic-oxic-settling-anoxic (A-OSA) process associated with the in-situ sludge reduction efficiency under synthetic landfill leachate treatment. The rates of excess sludge reduction with the inoculation of BM increased up to 53.6% (calculated as total suspended solids) and 38.3% (calculated as total volume), respectively. Side-stream reactors, as important components of the A-OSA process, were further studied to explore change of parameters related to in-situ sludge reduction. With the inoculation of BM, the release and conversion of extracellular polymeric substances and the dehydrogenase activity (increasing rate = 60.9%) were increased. Species richness and microbial diversity, as well as the microbial community composition (e.g., hydrolytic and fermentative bacteria), were improved via bioaugmentation. Moreover, potential gene functions of microorganisms were positively regulated and the abundance of gene expressions (e.g., nirK, norB) for in-situ sludge reduction could be improved.
AB - In-situ sludge reduction plays a significant role in reducing excess sludge production. This study investigated the role of beneficial microorganisms (BM) in the anoxic-oxic-settling-anoxic (A-OSA) process associated with the in-situ sludge reduction efficiency under synthetic landfill leachate treatment. The rates of excess sludge reduction with the inoculation of BM increased up to 53.6% (calculated as total suspended solids) and 38.3% (calculated as total volume), respectively. Side-stream reactors, as important components of the A-OSA process, were further studied to explore change of parameters related to in-situ sludge reduction. With the inoculation of BM, the release and conversion of extracellular polymeric substances and the dehydrogenase activity (increasing rate = 60.9%) were increased. Species richness and microbial diversity, as well as the microbial community composition (e.g., hydrolytic and fermentative bacteria), were improved via bioaugmentation. Moreover, potential gene functions of microorganisms were positively regulated and the abundance of gene expressions (e.g., nirK, norB) for in-situ sludge reduction could be improved.
KW - Beneficial microorganisms
KW - Microbial community
KW - Potential gene functions
KW - Side-stream reactor
KW - Sludge reduction
UR - http://www.scopus.com/inward/record.url?scp=85108405262&partnerID=8YFLogxK
U2 - 10.1016/j.jenvman.2021.113088
DO - 10.1016/j.jenvman.2021.113088
M3 - Article
C2 - 34174687
AN - SCOPUS:85108405262
SN - 0301-4797
VL - 295
JO - Journal of Environmental Management
JF - Journal of Environmental Management
M1 - 113088
ER -