Control of flowering time and cold response by a NAC-domain protein in Arabidopsis

So Yeon Yoo, Yunhee Kim, Soo Young Kim, Jong Seob Lee, Ji Hoon Ahn

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Background. Plants must integrate complex signals from environmental and endogenous cues to fine-tune the timing of flowering. Low temperature is one of the most common environmental stresses that affect flowering time; however, molecular mechanisms underlying the cold temperature regulation of flowering time are not fully understood. Methodology/Principal Findings. We report the identificatiori of a novel regulator, LONG VEGETATIVE PHASE 1 (LOV1), that controls flowering time and cold response. An Arabidopsis mutant, long vegetative phase 1-1D (lov1-1D) showing-the late-flowering phenotype, was isolated by activation tagging screening. Subsequent analyses demonstrated that the phenotype of the mutant resulted from the overexpression of a NAC-domain protein gene (At2g02450). Both gain- -ad loss-of-function alleles of LOV1 affected flowering time predominantly under long-day but not short-day conditions of suggesting that LOV1 may act within the photoperiod pathway. The expression of CONSTANS (CO), a floral promoter, was affected by LOV1 level, suggesting that LOV1 controls flowering time by negatively regulating CO expression. The epistatic relationship between CO and LOV1 was consistent with this proposed regulatory pathway. Physiological analyses to elucidate upstream signaling pathways revealed that LOV1 regulates the cold response in plants. Loss of LOV1 function resulted in hypersensitivity to cold temperature, whereas a gain-of-function allele conferred cold tolerance. The freezing tolerance was accompanied by upregulation of cold response genes, COLD-REGULATED 15A (COR15A) and COLD INDUCED 1 (KIN1) without affecting expression of the C-repeat-binding factor/dehydration responsive element-binding factor 1 (CBF/DREB1) family of genes. Conclusions. Our study shows that LOV1 functions as a floral repressor that negatively regulates CO expression under long-day conditions and acts as a common regulator of two intersecting pathways that regulate flowering time and the cold response, respectively. Our results suggest an overlapping pathway for controlling cold stress response and flowering time in plants.

Original languageEnglish
Article numbere642
JournalPLoS One
Volume2
Issue number7
DOIs
Publication statusPublished - 2007 Jul 25

Fingerprint

Arabidopsis Proteins
Arabidopsis
flowering
Proteins
proteins
Genes
Dehydration
Freezing
Temperature
Cold-Shock Response
cold tolerance
Alleles
Screening
Phenotype
Chemical activation
Photoperiod
alleles
Protein Domains
phenotype
mutants

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Control of flowering time and cold response by a NAC-domain protein in Arabidopsis. / Yoo, So Yeon; Kim, Yunhee; Kim, Soo Young; Lee, Jong Seob; Ahn, Ji Hoon.

In: PLoS One, Vol. 2, No. 7, e642, 25.07.2007.

Research output: Contribution to journalArticle

Yoo, So Yeon ; Kim, Yunhee ; Kim, Soo Young ; Lee, Jong Seob ; Ahn, Ji Hoon. / Control of flowering time and cold response by a NAC-domain protein in Arabidopsis. In: PLoS One. 2007 ; Vol. 2, No. 7.
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abstract = "Background. Plants must integrate complex signals from environmental and endogenous cues to fine-tune the timing of flowering. Low temperature is one of the most common environmental stresses that affect flowering time; however, molecular mechanisms underlying the cold temperature regulation of flowering time are not fully understood. Methodology/Principal Findings. We report the identificatiori of a novel regulator, LONG VEGETATIVE PHASE 1 (LOV1), that controls flowering time and cold response. An Arabidopsis mutant, long vegetative phase 1-1D (lov1-1D) showing-the late-flowering phenotype, was isolated by activation tagging screening. Subsequent analyses demonstrated that the phenotype of the mutant resulted from the overexpression of a NAC-domain protein gene (At2g02450). Both gain- -ad loss-of-function alleles of LOV1 affected flowering time predominantly under long-day but not short-day conditions of suggesting that LOV1 may act within the photoperiod pathway. The expression of CONSTANS (CO), a floral promoter, was affected by LOV1 level, suggesting that LOV1 controls flowering time by negatively regulating CO expression. The epistatic relationship between CO and LOV1 was consistent with this proposed regulatory pathway. Physiological analyses to elucidate upstream signaling pathways revealed that LOV1 regulates the cold response in plants. Loss of LOV1 function resulted in hypersensitivity to cold temperature, whereas a gain-of-function allele conferred cold tolerance. The freezing tolerance was accompanied by upregulation of cold response genes, COLD-REGULATED 15A (COR15A) and COLD INDUCED 1 (KIN1) without affecting expression of the C-repeat-binding factor/dehydration responsive element-binding factor 1 (CBF/DREB1) family of genes. Conclusions. Our study shows that LOV1 functions as a floral repressor that negatively regulates CO expression under long-day conditions and acts as a common regulator of two intersecting pathways that regulate flowering time and the cold response, respectively. Our results suggest an overlapping pathway for controlling cold stress response and flowering time in plants.",
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