Control of muscle differentiation by a mitochondria-targeted fluorophore

Yun Kyung Kim, Hyung Ho Ha, Jun Seok Lee, Xuezhi Bi, Young Hoon Ahn, Siti Hajar, Jae Jung Lee, Young Tae Chang

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

During muscle differentiation, mitochondria undergo dramatic changes in their morphology and distribution to prepare for the higher rate of energy consumption. By applying a mitochondria-targeted rosamine library in C2C12 myogenesis, we discovered one compound that controls muscle differentiation. When treated to undifferentiated myoblasts, our selected compound, B25, inhibited myotube formation, and when treated to fully differentiated myotubes, it induced fission of multinucleated myotubes into mononucleated fragments. Compared to myoseverin, which is known for inducing myotube fission by destabilizing microtubules, B25 affects neither microtubule stability nor cell cycle. Further investigation identified that B25 induces myotube fission through the activation of NF-κB, which is one of the important signaling pathways linked to skeletal muscle differentiation. So far, the use of small-molecule fluorophores is limited in the discovery of labeling agents or sensors. In addition to their potential as a sensor, here we show the application of fluorescent small molecules in the discovery of a bioactive probe that induces a specific cellular response.

Original languageEnglish
Pages (from-to)576-579
Number of pages4
JournalJournal of the American Chemical Society
Volume132
Issue number2
DOIs
Publication statusPublished - 2010 Jan 20
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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