Controlled Delivery of Stem Cell-Derived Trophic Factors Accelerates Kidney Repair After Renal Ischemia-Reperfusion Injury in Rats

Hyung Eun Yim, Doo Sang Kim, Hyun Chul Chung, Brian Shing, Kyung Hyun Moon, Sunil K. George, Michael W. Kim, Zachary Atala, Ji Hyun Kim, In Kap Ko, James J. Yoo

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Renal disease is a worldwide health issue. Besides transplantation, current therapies revolve around dialysis, which only delays disease progression but cannot replace other renal functions, such as synthesizing erythropoietin. To address these limitations, cell-based approaches have been proposed to restore damaged kidneys as an alternative to current therapies. Recent studies have shown that stem cell-derived secretomes can enhance tissue regeneration. However, many growth factors undergo rapid degradation when they are injected into the body in a soluble form. Efficient delivery and controlled release of secreting factors at the sites of injury would improve the efficacy in tissue regeneration. Herein, we developed a gel-based delivery system for controlled delivery of trophic factors in the conditioned medium (CM) secreted from human placental stem cells (HPSCs) and evaluated the effect of trophic factors on renal regeneration. CM treatment significantly enhanced cell proliferation and survival in vitro. Platelet-rich plasma (PRP) was used as a delivery vehicle for CM. Analysis of the release kinetics demonstrated that CM delivery through the PRP gel resulted in a controlled release of the factors both in vitro and in vivo. In an acute kidney injury model in rats, functional and structural analysis showed that CM delivery using the PRP gel system into the injured kidney minimized renal tissue damage, leading to a more rapid functional recovery when compared with saline, CM, or vehicle only injection groups. These results suggest that controlled delivery of HPSC-derived trophic factors may provide efficient repair of renal tissue injury. Stem Cells Translational Medicine 2019;8:959&970.

    Original languageEnglish
    Pages (from-to)959-970
    Number of pages12
    JournalStem Cells Translational Medicine
    Volume8
    Issue number9
    DOIs
    Publication statusPublished - 2019

    Keywords

    • Acute kidney injury
    • Conditioned medium
    • Drug delivery systems
    • Kidney regeneration

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

    Fingerprint

    Dive into the research topics of 'Controlled Delivery of Stem Cell-Derived Trophic Factors Accelerates Kidney Repair After Renal Ischemia-Reperfusion Injury in Rats'. Together they form a unique fingerprint.

    Cite this