Controlling molecular weight and degree of deacetylation of chitosan by response surface methodology

Kwon T. Hwang, Soon T. Jung, Gee D. Lee, Manjeet S. Chinnan, You S. Park, Hyun J. Park

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Response surface methodology (RSM) was used for controlling molecular weight (MW) and degree of deacetylation (DOD) of chitosan in chemical processing. In a reduced model, MW of chitosan is y= 1736166.406 - 250.745X1X2 - 265.452X2X3, with R2 = 0.86, and DOD of chitosan is y = 30.6069 + 0.3396X1 + 0.4948X2 + 0.0094X32, with R2 = 0.89. MW of chitosan depends on the crossproduct of temperature and NaOH concentration and the crossproduct of NaOH concentration and time, and DOD depends linearly on temperature and NaOH concentration, and quadratically on time. Chitosan was widely depolymerized in a range from 1,100 kDa to 100 kDa and deacetylated from 67.3 to 95.7% by NaOH alkaline treatment. MW and DOD of chitosan were drastically decreased and increased, respectively, with increase of temperature, reaction time, and NaOH concentration. Furthermore, the rate of MW decrease and DOD increase of chitosan gradually decreased with prolonged reaction time.

Original languageEnglish
Pages (from-to)1876-1882
Number of pages7
JournalJournal of agricultural and food chemistry
Volume50
Issue number7
DOIs
Publication statusPublished - 2002 Mar 27

Keywords

  • Chitosan
  • Degree of deacetylation
  • Molecular weight
  • Response surface methodology

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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