Convective exosome-tracing microfluidics for analysis of cell-non-autonomous neurogenesis

Hyun Jeong Oh, Yoojin Shin, Seok Chung, Do Won Hwang, Dong Soo Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effective role of exosome delivering neurogenic microRNA (miRNA) enables to induce efficient differentiation process during neurogenesis. The microfludic system capable of visualizing the exosomal behavior such as secretion, migration, and uptake of individual exosomes can be used as a robust technique to understand the exosome-mediated change of cellular behavior. Here, we developed the exosome-tracing microfluidic system to visualize exosomal transport carrying the neurogenic miRNA from leading to neighboring cells, and found a new mode of exosome-mediated cell-non-autonomous neurogenesis. The miR-193a facilitated neurogenesis in F11 cells by blocking proliferation-related target genes. In addition to time-lapse live-cell imaging using microfluidics visualized the convective transport of exosomes from differentiated to undifferentiated cells. Individual exosomes containing miR-193a from differentiated donor cells were taken up by undifferentiated cells to lead them to neurogenesis. Induction of anti-miR-193a was sufficient to block neurogenesis in F11 cells. Inhibition of the exosomal production by manumycin-A and treatment of anti-miR-193a in the differentiated donor cells failed to induce neurogenesis in undifferentiated recipient cells. These findings indicate that exosomes of neural progenitors and neurogenic miRNA within these exosomes propagate cell-non-autonomous differentiation to neighboring progenitors, to delineate the roles of exosome mediating neurogenesis of population of homologous neural progenitor cells.

Original languageEnglish
Pages (from-to)82-94
Number of pages13
JournalBiomaterials
Volume112
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Exosomes
Microfluidic Analytical Techniques
Neurogenesis
MicroRNAs
Microfluidics
Genes
Imaging techniques
Stem Cells
Cell Proliferation

Keywords

  • Cell-non-autonomous
  • Exosome imaging
  • Microfluidic platform
  • MiR-193a
  • Neurogenesis

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Convective exosome-tracing microfluidics for analysis of cell-non-autonomous neurogenesis. / Oh, Hyun Jeong; Shin, Yoojin; Chung, Seok; Hwang, Do Won; Lee, Dong Soo.

In: Biomaterials, Vol. 112, 01.01.2017, p. 82-94.

Research output: Contribution to journalArticle

Oh, Hyun Jeong ; Shin, Yoojin ; Chung, Seok ; Hwang, Do Won ; Lee, Dong Soo. / Convective exosome-tracing microfluidics for analysis of cell-non-autonomous neurogenesis. In: Biomaterials. 2017 ; Vol. 112. pp. 82-94.
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